Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,113,018	G→A	R84C (CGC→TGC)	ftsN ←	cell division protein FtsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,113,018	0	G	A	100.0%	77.0 / NA	20	R84C (CGC→TGC)	ftsN	cell division protein FtsN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/12);  total (8/12)

CATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAA  >  NZ_CP009273/4112893‑4113152                                                                                                                              |                                                                                                                                       cATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTtcttct                                                                                                                           >  2:353954/1‑139 (MQ=255)            gaagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTtggtgg                                                                                                                <  1:208428/139‑1 (MQ=255)                              gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGt                                                                                                 <  1:353954/136‑1 (MQ=255)                                   aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCt                                                                                         >  1:306506/1‑139 (MQ=255)                                            ggCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCa                                                                                <  2:576776/139‑1 (MQ=255)                                            ggCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCa                                                                                <  2:321413/139‑1 (MQ=255)                                                      cttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa                                                                      <  1:54036/139‑1 (MQ=255)                                                         caccacCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTCTCTGGCTTTGCAGCGTCTCGGAct                                                                    <  1:156998/138‑1 (MQ=255)                                                                 gCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCttctttct                                                           >  1:259255/1‑139 (MQ=255)                                                                           cTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                  <  1:5994/138‑1 (MQ=255)                                                                            tgtgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGt                                                 >  2:635721/1‑138 (MQ=255)                                                                              tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTa                                                <  1:384021/137‑1 (MQ=255)                                                                                ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg                                             <  1:726929/138‑1 (MQ=255)                                                                                ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg                                             <  1:9228/138‑1 (MQ=255)                                                                                               ggCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGa                             >  1:333753/1‑139 (MQ=255)                                                                                                gCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGa                             >  2:288608/1‑138 (MQ=255)                                                                                                     cGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                  >  2:183794/1‑112 (MQ=255)                                                                                                     cGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                  <  1:183794/112‑1 (MQ=255)                                                                                                                     tAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc          <  2:333753/136‑1 (MQ=255)                                                                                                                          tAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCaa  >  2:292975/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                       CATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAA  >  NZ_CP009273/4112893‑4113152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑