Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	303,602	T→G	N481T (AAC→ACC)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	303,602	0	T	G	100.0%	150.6 / NA	44	N481T (AAC→ACC)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/19);  total (25/19)

GACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCA  >  NZ_CP009273/303475‑303731                                                                                                                                 |                                                                                                                                  gACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTT‑AGGGTGTGTGTGCCGCCGATTGCACGGGTGTCGGCATCg                                                                                                                        >  1:201017/1‑139 (MQ=255)    aTTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTa                                                                                                                     <  1:26649/139‑1 (MQ=255)              tAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCAACCTTGTTGATGGTTCTAATTGTGGCCCGCCTAGACCACACTGTGTTC‑AGGTTGAAGGGGCCGGCCATTTCCCCGGGGCCGGCAACCCTACGTTCCAatt                                                                                                           >  1:334557/1‑139 (MQ=255)                     ccGTGTAATAATGGCTGTTGTAACGGCGGTCATTATTTTTGCTGGTGCCGGAAGTGCCCCGCTTCGACCACAGTGAGTTC‑TGGGTGGGGGGGGCGCCGGTTGGACGGGGGGTGGCATCGCCATGGCGCAatttgttgg                                                                                                     >  2:84080/1‑132 (MQ=255)                        tgtAATAATGGCTGTTGTAACGGCGGACATCAATGTTGCTGATGCTGAATGTGCCCAGCTTCGACCACAGCGAGGTC‑AGGGTGAGTGTGGCGGCGATTGGCCGGGTGTCGGCCACGCCTCGTCGCCATTGATTGTCa                                                                                                   >  1:649013/1‑137 (MQ=255)                          taataaTGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGTTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGc                                                                                               <  2:143507/139‑1 (MQ=255)                                  gCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGGGGGTTC‑AGGGTGAGGGTGCCGCCGGTTGCACGGGGGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGttttt                                                                                       >  1:280236/1‑139 (MQ=255)                                  gCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAAAGTGCCCAGCTTTGACCACAGTGAGGTT‑AGGGTTAGTGGGCCGCCGGTTGCACGGGGGTCGGCATCGCCACGGCGCAATTGATTGCCAAAGCGGGgtttt                                                                                       >  1:79105/1‑139 (MQ=255)                                       tgtAACGGCGGTCATCATTTTAGCTGGTGTTGAAAGTGCCCCGCTTTGCCCACAGAGAGGTCAAGGTTG‑GTGGGGCGCCCCTTGGGCGGGGGGCGGCCTGGCTCCGTGGCAATTTTTTTGCAACGCGGGGTTTTTTCTg                                                                                  >  2:225064/1‑139 (MQ=255)                                                ggTCATCATTGTAGCTGATGCTGGATGTGCCCAGCTTCGACCACAGTGGGTTC‑AGGGTGAGGGTGCCGCCGATTGCACGGGGGTCGGCCTCGCTACGTCGCAATTGATTGCCAATGCGGGGTTTTTTCTGGTTAAcccc                                                                         >  2:112546/1‑138 (MQ=255)                                                           tAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTa                                                               >  1:57082/1‑138 (MQ=255)                                                                gATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCt                                                         >  2:453813/1‑139 (MQ=255)                                                                 aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGcc                                                          <  1:112546/137‑1 (MQ=255)                                                                 aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCt                                                         <  2:649013/138‑1 (MQ=255)                                                                 aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTc                                                        <  2:79105/139‑1 (MQ=255)                                                                  tGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTcc                                                       <  1:452953/139‑1 (MQ=255)                                                                            gCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTg                                             >  2:588460/1‑139 (MQ=255)                                                                                         cacaGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGAtgctgc                                <  1:225064/139‑1 (MQ=255)                                                                                            aGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGa                             >  2:618898/1‑139 (MQ=255)                                                                                            aGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGa                             >  2:10035/1‑139 (MQ=255)                                                                                                 gTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCcaca                                                                       <  1:149750/92‑1 (MQ=255)                                                                                                 gTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCcaca                                                                       >  2:149750/1‑92 (MQ=255)                                                                                                  ttC‑AGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGAtgctgc                       >  1:469772/1‑139 (MQ=255)                                                                                                       ggTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTc                                                                                     <  1:315396/73‑1 (MQ=255)                                                                                                       ggTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTc                                                                                     >  2:315396/1‑73 (MQ=255)                                                                                                       ggTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCa                   <  1:134590/139‑1 (MQ=255)                                                                                                       ggTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCa                   >  1:156798/1‑139 (MQ=255)                                                                                                           gAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAg               >  1:10150/1‑139 (MQ=255)                                                                                                            aGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAg               <  1:669785/138‑1 (MQ=255)                                                                                                            aGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGc              <  1:84080/139‑1 (MQ=255)                                                                                                            aGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGc              <  1:745088/139‑1 (MQ=255)                                                                                                            aGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGc              <  2:276406/139‑1 (MQ=255)                                                                                                            aGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGc              <  1:453813/139‑1 (MQ=255)                                                                                                             gtgtGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGAt                            >  1:639873/1‑124 (MQ=255)                                                                                                             gtgtGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGAt                            <  2:639873/124‑1 (MQ=255)                                                                                                             gtgtGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCt             >  1:661094/1‑139 (MQ=255)                                                                                                                 gccgccGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCa         >  1:409217/1‑139 (MQ=255)                                                                                                                  ccgccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCAc        >  1:255030/1‑139 (MQ=255)                                                                                                                  ccgccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCAc        >  1:372283/1‑139 (MQ=255)                                                                                                                     ccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTa                                                  <  2:459084/94‑1 (MQ=255)                                                                                                                     ccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTa                                                  >  1:459084/1‑94 (MQ=255)                                                                                                                        aTTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCa  <  2:156798/139‑1 (MQ=255)                                                                                                                        aTTGCACGGGTGTCGACATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGAt                                                                                <  2:293912/61‑1 (MQ=255)                                                                                                                        aTTGCACGGGTGTCGACATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGAt                                                                                >  1:293912/1‑61 (MQ=255)                                                                                                                                 |                                                                                                                                  GACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTC‑AGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCA  >  NZ_CP009273/303475‑303731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑