Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,130,531	A→G	I540T (ATC→ACC)	ptsP ←	phosphoenolpyruvate‑‑protein phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,130,531	0	A	G	100.0%	181.6 / NA	47	I540T (ATC→ACC)	ptsP	phosphoenolpyruvate‑‑protein phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

GTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTT  >  NZ_CP009273/4130395‑4130654                                                                                                                                         |                                                                                                                            gTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCatggtg                                                                                                                           <  2:559715/139‑1 (MQ=255)  tGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGc                                                                                                                          >  2:30768/1‑139 (MQ=255)         tAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTg                                                                                                                   >  2:261408/1‑139 (MQ=255)                 gCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTtct                                                                                                           >  1:734908/1‑139 (MQ=255)                            aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTgg                                                                                                                  <  1:11711/121‑1 (MQ=255)                            aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTgg                                                                                                                  >  2:11711/1‑121 (MQ=255)                                 ccGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAAtgtg                                                                                           <  1:261408/139‑1 (MQ=255)                                   gCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGtt                                                                                         <  2:111333/139‑1 (MQ=255)                                   gCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGtt                                                                                         <  1:65390/139‑1 (MQ=255)                                       gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGt                                                                                      >  2:614580/1‑138 (MQ=255)                                       gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGt                                                                                      >  2:172104/1‑138 (MQ=255)                                                  gTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCatggtg                                                                                                                           <  2:308247/90‑1 (MQ=255)                                                  gTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCatggtg                                                                                                                           >  1:308247/1‑90 (MQ=255)                                                  gTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCt                                                                          >  1:108436/1‑139 (MQ=255)                                                  gTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCt                                                                          >  1:17797/1‑139 (MQ=255)                                                   tCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTg                                                                         >  1:507607/1‑139 (MQ=255)                                                           ttACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCtt                                                                 >  1:634957/1‑139 (MQ=255)                                                                 ccAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCt                                                                  <  1:204849/132‑1 (MQ=255)                                                                 ccAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCt                                                                  >  2:204849/1‑132 (MQ=255)                                                                         aaaGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttct                                                   >  1:67158/1‑139 (MQ=255)                                                                           aGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttcttt                                                 <  2:360343/139‑1 (MQ=255)                                                                               atcaatcTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCAcc                                             >  2:195166/1‑139 (MQ=255)                                                                                           gcagcaTTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  <  2:173841/138‑1 (MQ=255)                                                                                            cagcaTTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCGCCCATAAGATCTCtt                                <  1:204229/139‑1 (MQ=255)                                                                                            cagcaTTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCtt                                <  2:678536/139‑1 (MQ=255)                                                                                                  tGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAAt                                                                                              >  2:352730/1‑71 (MQ=255)                                                                                                  tGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAAt                                                                                              <  1:352730/71‑1 (MQ=255)                                                                                                  tGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttctt                                                  <  1:567772/115‑1 (MQ=255)                                                                                                  tGATCGATGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttctt                                                  >  2:567772/1‑115 (MQ=255)                                                                                                    aTCGACGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttcttt                                                 >  2:216647/1‑114 (MQ=255)                                                                                                    aTCGACGATGAACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttcttt                                                 <  1:216647/114‑1 (MQ=255)                                                                                                         tgatgaACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAg                                       >  2:394486/1‑119 (MQ=255)                                                                                                         tgatgaACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAg                                       <  1:394486/119‑1 (MQ=255)                                                                                                             gaACATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGatcatc               >  2:17757/1‑139 (MQ=255)                                                                                                              aaCATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCtcat                                                                                                        >  1:599944/1‑49 (MQ=255)                                                                                                              aaCATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCtcat                                                                                                        <  2:599944/49‑1 (MQ=255)                                                                                                              aaCATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCtcat                                                                                                        >  1:435754/1‑49 (MQ=255)                                                                                                              aaCATCACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCtcat                                                                                                        <  2:435754/49‑1 (MQ=255)                                                                                                                   cACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTc                               <  1:506261/117‑1 (MQ=255)                                                                                                                   cACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTc                               >  2:506261/1‑117 (MQ=255)                                                                                                                   cACCGACGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGAt          >  2:406902/1‑138 (MQ=255)                                                                                                                        aCGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  <  2:689657/109‑1 (MQ=255)                                                                                                                        aCGGCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  >  1:689657/1‑109 (MQ=255)                                                                                                                          ggCACTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGAttt  >  2:383749/1‑139 (MQ=255)                                                                                                                             aCTTCCAGCATGGTGCTGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGc                                                             <  2:320492/77‑1 (MQ=255)                                                                                                                             aCTTCCAGCATGGTGCTGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGc                                                             >  1:320492/1‑77 (MQ=255)                                                                                                                             aCTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  <  1:130700/104‑1 (MQ=255)                                                                                                                             aCTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  >  2:106211/1‑104 (MQ=255)                                                                                                                             aCTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  <  1:106211/104‑1 (MQ=255)                                                                                                                             aCTTCCAGCATGGTGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                                  >  2:130700/1‑104 (MQ=255)                                                                                                                                         |                                                                                                                            GTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTT  >  NZ_CP009273/4130395‑4130654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑