| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,171,129 | T→C | intergenic (+276/‑44) | rplL → / → rpoB | 50S ribosomal protein L7/L12/DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,171,129 | 0 | T | C | 95.8% | 168.5 / ‑1.3 | 48 | intergenic (+276/‑44) | rplL/rpoB | 50S ribosomal protein L7/L12/DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (23/23); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATC > NZ_CP009273/4171003‑4171262 | atatACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGAt < 1:653533/139‑1 (MQ=255) tGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCa < 1:101903/123‑1 (MQ=255) tGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCa > 2:101903/1‑123 (MQ=255) tGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTc > 1:17987/1‑138 (MQ=255) tGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTc > 1:431571/1‑138 (MQ=255) tGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTc > 2:754639/1‑138 (MQ=255) aCAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACtt > 1:83100/1‑139 (MQ=255) cAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTg > 1:395910/1‑139 (MQ=255) tCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGc > 2:166756/1‑137 (MQ=255) tgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAg < 2:83100/136‑1 (MQ=255) tgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAgg < 2:572133/137‑1 (MQ=255) tgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAg < 2:285374/136‑1 (MQ=255) tgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGaa > 2:169884/1‑139 (MQ=255) tgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGaa < 1:754639/139‑1 (MQ=255) aaTCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCt < 1:166756/139‑1 (MQ=255) aaTCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCt < 1:169884/139‑1 (MQ=255) aTCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTa < 1:353004/139‑1 (MQ=255) aaTGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAg > 2:235207/1‑126 (MQ=255) aaTGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAg < 1:235207/126‑1 (MQ=255) aaTGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGtt < 2:395910/139‑1 (MQ=255) aaTGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGtt > 1:483632/1‑139 (MQ=255) aaTGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGtt < 1:277942/139‑1 (MQ=255) aaTGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGtt > 2:277942/1‑139 (MQ=255) tGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTa < 1:638747/139‑1 (MQ=255) aGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATAcc > 2:7478/1‑137 (MQ=255) tGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGt < 2:485125/88‑1 (MQ=255) tGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGt > 1:485125/1‑88 (MQ=255) aTAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGaaaaa < 2:17987/139‑1 (MQ=255) aTAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGaaaa > 2:163107/1‑138 (MQ=255) aTAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGaaaa > 2:203543/1‑138 (MQ=255) ggCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCg > 1:349688/1‑139 (MQ=255) aTTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAg < 2:151797/89‑1 (MQ=255) aTTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAg > 1:151797/1‑89 (MQ=255) ttGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACa < 1:114688/66‑1 (MQ=255) ttGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACa > 2:114688/1‑66 (MQ=255) tGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAACGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAg > 1:386354/1‑138 (MQ=255) ttCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTaa < 1:364790/138‑1 (MQ=255) tCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGa > 1:89622/1‑48 (MQ=255) tCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCCCAATGGACAGa < 2:89622/48‑1 (MQ=255) gtgtTGCACAAACTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCt < 2:483632/139‑1 (MQ=255) tgctGCACGAACTGCACGCCCACTGGATAGATGGGTAGACTTGACAGCGAGCTGAGGTACCCCATGGTTTACTCTTATACCGAGCAATAACGTATTCGTAAGGATTTTGGTTAACGTCCACAAGTTCTGGATGTACCt > 2:186269/4‑138 (MQ=255) aaCTGTCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCttt < 1:7478/139‑1 (MQ=255) tCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGtt < 2:508961/55‑1 (MQ=255) tCCGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGtt > 1:508961/1‑55 (MQ=255) tCCGCCCAACGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTAt < 2:386354/138‑1 (MQ=255) ccGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTa > 2:560632/1‑49 (MQ=255) ccGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTa < 1:560632/49‑1 (MQ=255) cGCCCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATc > 2:656412/1‑137 (MQ=255) | ATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATC > NZ_CP009273/4171003‑4171262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |