Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,320,763	T→C	S149P (TCG→CCG)	proP →	glycine betaine/L‑proline transporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,320,763	0	T	C	100.0%	186.0 / NA	51	S149P (TCG→CCG)	proP	glycine betaine/L‑proline transporter ProP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/22);  total (29/22)

TGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGGCGAAGC  >  NZ_CP009273/4320635‑4320896                                                                                                                                 |                                                                                                                                       tGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                                               <  2:137672/138‑1 (MQ=255)  gTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgtt                                                                                                                             >  2:121084/1‑139 (MQ=255)   tCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                            <  1:614628/139‑1 (MQ=255)               ttCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTccc                                                                                                                <  1:708792/139‑1 (MQ=255)                     aTTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑cc                                                                                                         >  2:326239/1‑139 (MQ=255)                         gCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTa                                                                                                      >  2:475502/1‑138 (MQ=255)                              aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                            <  2:666427/112‑1 (MQ=255)                              aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                            >  1:666427/1‑112 (MQ=255)                              aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTg                                                                                                >  2:604392/1‑139 (MQ=255)                                   gTCCTACGACACGATTGGTATTTGGGCATCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTcccc                                                                                                               <  2:243125/120‑1 (MQ=255)                                   gTCCTACGACACGATTGGTATTTGGGCATCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTcccc                                                                                                               >  1:243125/1‑120 (MQ=255)                                                aTTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGTCACAAGGTTTGTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCTTAAACGTGGCTTTATGGGCAgctggc                                                                              <  2:547602/139‑1 (MQ=255)                                                aTTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAgctggc                                                                              >  1:584575/1‑139 (MQ=255)                                                     tATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGAc                                                                         <  1:604392/139‑1 (MQ=255)                                                              aCCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTc                                                                 >  1:38179/1‑138 (MQ=255)                                                               ccGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTa                                                               >  2:7623/1‑139 (MQ=255)                                                               ccGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTa                                                               >  1:580278/1‑139 (MQ=255)                                                               ccGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTa                                                               >  2:407494/1‑139 (MQ=255)                                                                             gATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAAt                                                                                                                      <  1:748161/71‑1 (MQ=255)                                                                             gATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAAt                                                                                                                      >  2:748161/1‑71 (MQ=255)                                                                             gATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACtt                                                                       >  1:243704/1‑118 (MQ=255)                                                                             gATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACtt                                                                       <  2:243704/118‑1 (MQ=255)                                                                              aTCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTtgtg                                                 <  1:519402/138‑1 (MQ=255)                                                                                  gTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTggg                                            >  1:151599/1‑139 (MQ=255)                                                                                   tAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                                               <  2:363452/135‑1 (MQ=255)                                                                                   tAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                                               <  2:186876/135‑1 (MQ=255)                                                                                     aGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGat                                                                                                                                    <  1:401039/49‑1 (MQ=255)                                                                                     aGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGat                                                                                                                                    >  2:401039/1‑49 (MQ=255)                                                                                          gCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTccc                                                                                                                >  1:618159/1‑64 (MQ=255)                                                                                          gCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTccc                                                                                                                <  2:618159/64‑1 (MQ=255)                                                                                           cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGt                                                      >  2:768673/1‑120 (MQ=255)                                                                                           cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGt                                                      <  1:768673/120‑1 (MQ=255)                                                                                           cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTg                                          >  2:750663/1‑132 (MQ=255)                                                                                           cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTg                                          <  1:750663/132‑1 (MQ=255)                                                                                           cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCgt                                   >  1:584382/1‑139 (MQ=255)                                                                                           cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCgt                                   >  1:761435/1‑139 (MQ=255)                                                                                             caAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCgtgg                                 >  1:100792/1‑139 (MQ=255)                                                                                             caAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCgtgg                                 <  2:633175/139‑1 (MQ=255)                                                                                                 gTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                                                              >  1:188592/1‑59 (MQ=255)                                                                                                 gTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                                                              <  2:188592/59‑1 (MQ=255)                                                                                                     ctcGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGtt                          >  1:174744/1‑138 (MQ=255)                                                                                                                        aCCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCgg                                       >  1:759798/1‑106 (MQ=255)                                                                                                                        aCCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCgg                                       <  2:759798/106‑1 (MQ=255)                                                                                                                         ccGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcg      <  2:266082/138‑1 (MQ=255)                                                                                                                           ggggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcgaag   >  2:508946/1‑139 (MQ=255)                                                                                                                             ggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCa                                                                                    <  2:13734/56‑1 (MQ=255)                                                                                                                             ggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCa                                                                                    >  1:13734/1‑56 (MQ=255)                                                                                                                             ggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                                               <  1:604845/93‑1 (MQ=255)                                                                                                                             ggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                                               >  2:604845/1‑93 (MQ=255)                                                                                                                             ggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcgaagc  >  1:19231/1‑138 (MQ=255)                                                                                                                             ggCGCCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcgaagc  >  2:318122/1‑138 (MQ=255)                                                                                                                                 |                                                                                                                                       TGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATATTTGTTGCGGAATACTCCCCTGA‑CCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGGCGAAGC  >  NZ_CP009273/4320635‑4320896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑