Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,355,460	A→C	G377G (GGT→GGG)	dcuA ←	anaerobic C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,355,460	0	A	C	100.0%	161.7 / NA	42	G377G (GGT→GGG)	dcuA	anaerobic C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/16);  total (26/16)

AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGC  >  NZ_CP009273/4355344‑4355591                                                                                                                     |                                                                                                                                    agagTACCCGGGATGAAGAACGGATGGCTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAaagaag                                                                                                               >  1:105465/1‑139 (MQ=255)  gagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAaagaag                                                                                                               >  2:280934/1‑138 (MQ=255)           ggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAAcagcggt                                                                                                     >  1:245707/1‑139 (MQ=255)                                 gACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGc                                                                                >  1:88521/1‑138 (MQ=255)                                  aCGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCa                                                                              <  1:747861/139‑1 (MQ=255)                                      aTTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGc                                                                          >  1:763487/1‑139 (MQ=255)                                      aTTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGc                                                                          >  1:437535/1‑139 (MQ=255)                                                aCGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAaaga                                                                                                                 <  1:322315/90‑1 (MQ=255)                                                aCGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAaaga                                                                                                                 >  2:322315/1‑90 (MQ=255)                                                          cccGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTg                                                      <  1:387263/139‑1 (MQ=255)                                                            cGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCg                                                    >  2:138724/1‑139 (MQ=255)                                                             gtgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATTAGTGCTTTTGCgg                                                   >  1:64691/1‑139 (MQ=255)                                                             gtgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCgg                                                   >  1:457804/1‑139 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAAcagcggt                                                                                                     >  1:262913/1‑88 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAAcagcggt                                                                                                     <  2:262913/88‑1 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGt                                                  >  2:508352/1‑139 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGt                                                  <  2:565136/139‑1 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGt                                                  >  2:142158/1‑139 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGt                                                  >  1:694926/1‑139 (MQ=255)                                                              tgtCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGt                                                  >  1:380669/1‑139 (MQ=255)                                                                tCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATTAGTGCTTTTGCGGTTg                                                <  2:64691/139‑1 (MQ=255)                                                                  atccatcTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCGGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCAt                                                                 <  2:645044/120‑1 (MQ=255)                                                                  atccatcTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCGGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCAt                                                                 >  1:645044/1‑120 (MQ=255)                                                                  atccatcTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTc                                                                                     >  1:319671/1‑100 (MQ=255)                                                                  atccatcTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTc                                                                                     <  2:319671/100‑1 (MQ=255)                                                                      atcTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCt                                          <  2:380669/139‑1 (MQ=255)                                                                           tACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTga                                        <  2:88521/136‑1 (MQ=255)                                                                           tACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagag                                     >  1:204106/1‑139 (MQ=255)                                                                             ccGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTa                                   <  1:280934/139‑1 (MQ=255)                                                                                cagcaACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcag                                >  1:497404/1‑139 (MQ=255)                                                                                cagcaACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcag                                <  2:308335/139‑1 (MQ=255)                                                                                cagcaACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcag                                <  1:138724/139‑1 (MQ=255)                                                                                cagcaACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGGGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagagaa                                   >  2:109460/1‑134 (MQ=255)                                                                                  gcaACCAGCGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcag                                >  1:580936/1‑137 (MQ=255)                                                                                          cGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAAcagcggt                                                                                                     >  1:13761/1‑60 (MQ=255)                                                                                          cGTCGGGTAGGTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAAcagcggt                                                                                                     <  2:13761/60‑1 (MQ=255)                                                                                                    gTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAaagaag             >  1:628982/1‑138 (MQ=255)                                                                                                    gTCGGCAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAaagaag             >  1:14677/1‑138 (MQ=255)                                                                                                         cAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg                                                >  1:668234/1‑98 (MQ=255)                                                                                                         cAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg                                                <  2:668234/98‑1 (MQ=255)                                                                                                             aTGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACgg   <  2:763487/139‑1 (MQ=255)                                                                                                              tGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACGGCAGAGCAGAAGCAACGAAGAAGATGACGGc  >  2:98382/1‑139 (MQ=255)                                                                                                                     |                                                                                                                                    AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGC  >  NZ_CP009273/4355344‑4355591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑