Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,389,340	A→G	E91G (GAA→GGA)	miaA →	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,389,340	0	A	G	100.0%	129.4 / NA	37	E91G (GAA→GGA)	miaA	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

GGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACA  >  NZ_CP009273/4389214‑4389475                                                                                                                               |                                                                                                                                        ggatggatATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGatat                                                                                                                             <  2:53771/139‑1 (MQ=255) ggatggatATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGatat                                                                                                                             >  1:574607/1‑139 (MQ=255)    tggatATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCAc                                                                                                                          >  2:88680/1‑139 (MQ=255)          tCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCgcgg                                                                                                                     <  2:574607/138‑1 (MQ=255)           cGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCgcggcg                                                                                                                   >  1:593010/1‑139 (MQ=255)            gggACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCgcggcg                                                                                                                   <  1:88680/138‑1 (MQ=255)                 ggCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGGTATCACCGCggcggggcgg                                                                                                             >  2:723896/1‑139 (MQ=255)                 ggCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCggcggggcg                                                                                                              >  1:312859/1‑138 (MQ=255)                  gCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGa                                                                                                            <  2:393836/139‑1 (MQ=255)                   cGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGAt                                                                                                           >  1:230289/1‑139 (MQ=255)                   cGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGAt                                                                                                           <  2:15020/139‑1 (MQ=255)                   cGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAAGGGCCGGTATCACCGAggcgggggcgaa                                                                                                           >  2:238272/1‑134 (MQ=255)                           aaCGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGt                                                                                                   <  1:356436/139‑1 (MQ=255)                                  aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            <  2:749602/139‑1 (MQ=255)                                  aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            <  1:723896/139‑1 (MQ=255)                                       ttACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTa                                                                                       <  2:312859/139‑1 (MQ=255)                                        tACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCGTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAGAGGCCGGTATCAACGCGGGGGAGAGGGTTCCACGTTTTATGGGCGGt                                                                                        >  2:57372/1‑137 (MQ=255)                                          cTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACg                                                                                     <  1:652442/138‑1 (MQ=255)                                               cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            >  1:581018/1‑126 (MQ=255)                                               cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            <  2:581018/126‑1 (MQ=255)                                               cgcgccgcACCGATTGCTGGATATCCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTCTCGCCGCGATGCGCTGGCGTGAATGGCCGATATCACCGCGGCGGGGCGGAGCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               <  2:260637/139‑1 (MQ=255)                                                 cgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTa                                                                             >  1:540381/1‑139 (MQ=255)                                                            ttGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCAt                                                                  <  2:540381/139‑1 (MQ=255)                                                            ttGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCAt                                                                  <  2:230289/139‑1 (MQ=255)                                                                       tCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               >  2:165501/1‑115 (MQ=255)                                                                       tCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               <  1:165501/115‑1 (MQ=255)                                                                       tCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAggg                                                       >  1:488364/1‑139 (MQ=255)                                                                       tCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAggg                                                       >  2:688750/1‑139 (MQ=255)                                                                                 tCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               <  2:518553/105‑1 (MQ=255)                                                                                 tCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               >  1:518553/1‑105 (MQ=255)                                                                                 tCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGGACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTcgccgc                                             >  2:725389/1‑139 (MQ=255)                                                                                           aCTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGGACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCgg                                    >  1:141225/1‑138 (MQ=255)                                                                                            cTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGc                                   <  2:55182/138‑1 (MQ=255)                                                                                            cTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCa                                  <  2:592578/139‑1 (MQ=255)                                                                                                 ctgctgATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGAccc                             >  1:222868/1‑139 (MQ=255)                                                                                                                       gcTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAg       <  1:114925/139‑1 (MQ=255)                                                                                                                            cGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACa  <  1:688750/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        GGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACA  >  NZ_CP009273/4389214‑4389475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑