Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,401,268	T→G	G50G (GGT→GGG)	yjfK →	YjfK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,401,268	0	T	G	100.0%	79.8 / NA	22	G50G (GGT→GGG)	yjfK	YjfK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/14);  total (8/14)

CAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGA  >  NZ_CP009273/4401134‑4401390                                                                                                                                       |                                                                                                                           cAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                         <  2:261399/138‑1 (MQ=255)              gCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                           <  1:51637/139‑1 (MQ=255)                       ataaGCCAGCTATCGCTCGTGGTCCGCTGTGACTTCATCTCAATAGTGGCTTTACGCTCTACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAg                                                                                                  <  1:720356/139‑1 (MQ=255)                       ataaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAg                                                                                                  <  1:570397/139‑1 (MQ=255)                            ccAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCcaca                                                                                             <  1:205516/139‑1 (MQ=255)                                cTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGa                                                                                         >  2:227536/1‑139 (MQ=255)                                                       cTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGAttt                                                                  <  1:420910/139‑1 (MQ=255)                                                          cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                                                                <  1:168476/138‑1 (MQ=255)                                                          cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                                                                <  1:198497/138‑1 (MQ=255)                                                                 aTAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGctacta                                                        <  2:463662/139‑1 (MQ=255)                                                                      ggCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                         >  1:177486/1‑69 (MQ=255)                                                                      ggCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                         <  2:177486/69‑1 (MQ=255)                                                                       gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACtt                                                   >  2:749810/1‑138 (MQ=255)                                                                          ttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                           >  1:413656/1‑79 (MQ=255)                                                                          ttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                           <  2:413656/79‑1 (MQ=255)                                                                                              tttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATc                            >  1:717751/1‑138 (MQ=255)                                                                                                 cgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATa                        >  2:112152/1‑139 (MQ=255)                                                                                                         ggAAGATGAATTGCTGATTGCGATGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACcggc                >  2:455423/1‑139 (MQ=255)                                                                                                             gATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGgcggcg            <  1:166621/139‑1 (MQ=255)                                                                                                              aTGAATTGCGGAGTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGa           <  2:39911/139‑1 (MQ=255)                                                                                                                 aaTTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGgcggcg            >  1:117244/1‑135 (MQ=255)                                                                                                                         aTTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtga  <  2:422334/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                           CAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGA  >  NZ_CP009273/4401134‑4401390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑