Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,498,429	T→G	R2S (AGA→AGC)	BW25113_RS22240 ←	IS30‑like element IS30 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,498,429	0	T	G	100.0%	98.5 / NA	31	R2S (AGA→AGC)	BW25113_RS22240	IS30‑like element IS30 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/14);  total (17/14)

CACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTT  >  NZ_CP009273/4498362‑4498563                                                                    |                                                                                                                                       cACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTg                                                                 >  1:496269/1‑139 (MQ=14)   cTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCt                                                               <  2:167803/139‑1 (MQ=18)      aaGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCtttt                                                            >  1:232351/1‑139 (MQ=21)       aGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCttttt                                                           <  2:735394/139‑1 (MQ=21)             ttCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAata                                                     >  1:633663/1‑139 (MQ=21)             ttCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAat                                                      <  1:444723/138‑1 (MQ=21)                cGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAatatat                                                  <  2:56551/139‑1 (MQ=37)                  ttcttcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAtct                                                >  1:715940/1‑139 (MQ=38)                  ttcttcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAtct                                                <  1:110535/139‑1 (MQ=38)                  ttcttcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAtct                                                >  2:134182/1‑139 (MQ=38)                  ttcttcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAtct                                                >  1:479526/1‑139 (MQ=38)                  ttcttcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAtct                                                >  1:231651/1‑139 (MQ=38)                  ttcttcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTCTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAtct                                                <  2:543646/139‑1 (MQ=255)                       ccATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAat                                                      >  1:331658/1‑128 (MQ=25)                       ccATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAat                                                      <  2:331658/128‑1 (MQ=25)                       ccATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCt                                            >  2:11840/1‑138 (MQ=255)                                aaaaaCAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTT‑ATATATCTCGCTCAAGGCGAGCt                                 <  1:659052/139‑1 (MQ=255)                                 aaaaCAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCt                                 >  1:152238/1‑139 (MQ=255)                                       gagGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCAtt                            >  1:256864/1‑138 (MQ=255)                                       gagGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCAtt                            >  2:429220/1‑138 (MQ=255)                                        agGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTa                          <  1:496610/139‑1 (MQ=255)                                              tttCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGcc                     <  1:134182/138‑1 (MQ=255)                                                     tgctgTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGc               <  2:13961/137‑1 (MQ=255)                                                      gctgTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTa                          >  1:612467/1‑125 (MQ=255)                                                      gctgTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTa                          <  2:612467/125‑1 (MQ=255)                                                                 tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAGTTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGcc                     <  2:124548/119‑1 (MQ=255)                                                                 tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGcc                     >  1:124548/1‑119 (MQ=255)                                                                 tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTg          <  1:72011/130‑1 (MQ=255)                                                                 tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTg          >  2:72011/1‑130 (MQ=255)                                                                 tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt  >  2:527983/1‑138 (MQ=255)                                                                 tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt  >  1:114796/1‑138 (MQ=255)                                                                    |                                                                                                                                       CACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTT  >  NZ_CP009273/4498362‑4498563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑