Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	338,646	A→T	M18L (ATG→TTG)	yahK →	NADPH‑dependent aldehyde reductase YahK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	338,646	0	A	T	100.0%	168.8 / NA	43	M18L (ATG→TTG)	yahK	NADPH‑dependent aldehyde reductase YahK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/18);  total (25/18)

TGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGCCGGGTCATG  >  NZ_CP009273/338513‑338781                                                                                                                                      |                                                                                                                                        tGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGa                                                                                                                                     >  2:154096/1‑138 (MQ=255)  ggCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGata                                                                                                                                   >  1:419145/1‑139 (MQ=255)       tatTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCTCTAAACAACCACTTGAACCGTTGGATATCAcc                                                                                                                              <  1:675068/139‑1 (MQ=255)        atTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCAccc                                                                                                                             >  1:462587/1‑139 (MQ=255)             aTGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCg                                                                                                                         <  1:753852/138‑1 (MQ=255)             aTGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCg                                                                                                                         >  2:753852/1‑138 (MQ=255)             aTGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGt                                                                                                                        >  1:135810/1‑139 (MQ=255)                 aCGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAAc                                                                                                                    >  1:524905/1‑139 (MQ=255)                 aCGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAAc                                                                                                                    >  1:593682/1‑139 (MQ=255)                   gcgTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACAACTTGAACCGTTGGATATCACCCGGCGTGAACCg                                                                                                                  >  2:577292/1‑139 (MQ=255)                                           ggCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcga                                                                                           >  2:237953/1‑138 (MQ=255)                                               ttGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcgaaatcg                                                                                      >  1:254093/1‑139 (MQ=255)                                                                cTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGc                                                                     >  1:719217/1‑139 (MQ=255)                                                                   aTCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcga                                                                                           <  1:97856/114‑1 (MQ=255)                                                                   aTCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcga                                                                                           >  2:97856/1‑114 (MQ=255)                                                                   aTCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCAt                                                                  >  2:759772/1‑139 (MQ=255)                                                                        gAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGa                                                             >  1:611811/1‑139 (MQ=255)                                                                        gAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGa                                                             >  2:543009/1‑139 (MQ=255)                                                                          gTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc                                                           <  1:26666/139‑1 (MQ=255)                                                                               cacaATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                                                      <  2:611811/139‑1 (MQ=255)                                                                               cacaATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                                                      <  2:265430/139‑1 (MQ=255)                                                                                  aaTGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAg                                                   >  2:676150/1‑139 (MQ=255)                                                                                          tCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTc                                           >  1:213077/1‑139 (MQ=255)                                                                                            aaaGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc                                                           <  1:379140/121‑1 (MQ=255)                                                                                            aaaGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc                                                           >  2:379140/1‑121 (MQ=255)                                                                                            aaaGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTcc                                          >  2:641436/1‑138 (MQ=255)                                                                                              aGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAg                                       >  1:749657/1‑139 (MQ=255)                                                                                              aGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAg                                       <  1:527278/139‑1 (MQ=255)                                                                                                   ttGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGc                                  >  1:116859/1‑139 (MQ=255)                                                                                                     ggTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCgg                                >  1:82417/1‑139 (MQ=255)                                                                                                      gTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCggg                               <  1:543009/139‑1 (MQ=255)                                                                                                         cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                             <  2:213077/138‑1 (MQ=255)                                                                                                         cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                             <  2:135810/138‑1 (MQ=255)                                                                                                         cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                             <  1:759772/138‑1 (MQ=255)                                                                                                         cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                             <  2:719217/138‑1 (MQ=255)                                                                                                                 gCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGa                                                                                                           <  1:757701/52‑1 (MQ=255)                                                                                                                 gCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGa                                                                                                           >  2:757701/1‑52 (MQ=255)                                                                                                                   tAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                                                      <  2:598053/103‑1 (MQ=255)                                                                                                                   tAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                                                      >  1:598053/1‑103 (MQ=255)                                                                                                                     aacaacCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCggg                               >  2:111232/1‑124 (MQ=255)                                                                                                                     aacaacCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCggg                               <  1:111232/124‑1 (MQ=255)                                                                                                                                 aaCCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGCCGGGTCa    <  2:82417/139‑1 (MQ=255)                                                                                                                                   ccGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGACCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGCCGGGTCATg  <  2:419145/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGCCGGGTCATG  >  NZ_CP009273/338513‑338781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑