Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,507,805 T→C E83G (GAA→GGA)  fecI ← RNA polymerase sigma factor FecI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,507,8050TC100.0% 200.2 / NA 53E83G (GAA→GGA) fecIRNA polymerase sigma factor FecI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

TCACGTGTTTTGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGG  >  NZ_CP009273/4507673‑4507927
                                                                                                                                    |                                                                                                                          
tCACGTGTTTTGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAgcg                                                                                                                      >  2:514444/1‑139 (MQ=255)
tCACGTGTTTTGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAgcg                                                                                                                      >  1:278503/1‑139 (MQ=255)
       ttttGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCg                                                                                                               >  2:401721/1‑139 (MQ=255)
         ttGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCgg                                                                                                              <  1:308775/138‑1 (MQ=255)
         ttGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGc                                                                                                             >  1:516736/1‑139 (MQ=255)
              gTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAAc                                                                                                        <  2:55234/139‑1 (MQ=255)
                         ccAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCAt                                                                                             >  1:43531/1‑139 (MQ=255)
                           aGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCa                                                                                           <  2:16714/139‑1 (MQ=255)
                             cATGCTGTCGAGGAGTTGTAGGGTCTAGAGTTGGCTTTCGCGTTCCTCAGGTGACGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAcg                                                                                         <  1:82825/139‑1 (MQ=255)
                                  tGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGaaa                                                                                                         >  1:146376/1‑118 (MQ=255)
                                  tGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGaaa                                                                                                         <  2:146376/118‑1 (MQ=255)
                                  tGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTcc                                                                                                  >  2:599521/1‑125 (MQ=255)
                                  tGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTcc                                                                                                  <  1:599521/125‑1 (MQ=255)
                                  tGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGttt                                                                                    >  1:391851/1‑139 (MQ=255)
                                       aggagTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa                                                                               <  1:272341/139‑1 (MQ=255)
                                              gTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGaaa                                                                                                         >  2:194521/1‑106 (MQ=255)
                                              gTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGaaa                                                                                                         <  1:194521/106‑1 (MQ=255)
                                               tAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCag                                                                       <  1:577018/139‑1 (MQ=255)
                                                    tctcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAcgcg                                                                                       <  1:304625/118‑1 (MQ=255)
                                                    tctcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAcgcg                                                                                       >  2:304625/1‑118 (MQ=255)
                                                    tctcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGaggaa                                                                  <  2:764967/139‑1 (MQ=255)
                                                    tctcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGaggaa                                                                  <  1:237947/139‑1 (MQ=255)
                                                    tctcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGagg                                                                    >  2:536763/1‑137 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGa                                                                                                           >  1:43295/1‑96 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGa                                                                                                           <  2:43295/96‑1 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTcca                                                                                                 >  1:249394/1‑106 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTcca                                                                                                 <  2:249394/106‑1 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTcc                                                                                                  <  1:459360/105‑1 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTcc                                                                                                  >  2:459360/1‑105 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGttt                                                                                    <  2:386299/119‑1 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGttt                                                                                    >  1:386299/1‑119 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGCTAGTGCAGaggaagg                                                                >  2:670803/1‑139 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGaggaag                                                                 >  2:697299/1‑138 (MQ=255)
                                                      tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGaggaag                                                                 >  1:639851/1‑138 (MQ=255)
                                                        gAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGaggaagga                                                               <  2:43531/138‑1 (MQ=255)
                                                          gTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCg                                                            <  2:516736/139‑1 (MQ=255)
                                                          gTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCg                                                            <  1:662129/139‑1 (MQ=255)
                                                           ttGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGa                                                           <  1:726110/139‑1 (MQ=255)
                                                                   cgcgTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATcgcg                                                   >  2:242729/1‑139 (MQ=255)
                                                                                    ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa                                                                               >  2:736265/1‑94 (MQ=255)
                                                                                    ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa                                                                               <  1:736265/94‑1 (MQ=255)
                                                                                      cgcTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCg                                <  1:536763/139‑1 (MQ=255)
                                                                                         tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGt                                                                                                                   <  2:404488/53‑1 (MQ=255)
                                                                                         tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGt                                                                                                                   >  1:404488/1‑53 (MQ=255)
                                                                                         tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGt                                                                           >  1:359854/1‑93 (MQ=255)
                                                                                         tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGt                                                                           <  2:359854/93‑1 (MQ=255)
                                                                                         tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCt                              >  1:74849/1‑138 (MQ=255)
                                                                                                       tAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGt                                                                                                    >  2:86773/1‑54 (MQ=255)
                                                                                                       tAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGt                                                                                                    <  1:86773/54‑1 (MQ=255)
                                                                                                           cgcCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCaaaaaa           <  2:500593/139‑1 (MQ=255)
                                                                                                               aGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAgtgt       >  2:630543/1‑139 (MQ=255)
                                                                                                                gCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTc      <  1:401721/139‑1 (MQ=255)
                                                                                                                       cAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTgg  <  1:762250/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                          
TCACGTGTTTTGCCGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGG  >  NZ_CP009273/4507673‑4507927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑