| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,589,054 | T→G | T69P (ACC→CCC) | opgB ← | phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,589,054 | 0 | T | G | 97.9% | 167.0 / ‑2.9 | 47 | coding (205/2292 nt) | opgB | phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (19/27); minor base . (0/1); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
CTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAG > NZ_CP009273/4588927‑4589184 | cTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTgg < 1:406910/139‑1 (MQ=255) tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGtt < 2:482487/138‑1 (MQ=255) tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa < 1:289654/139‑1 (MQ=255) tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa < 1:451392/139‑1 (MQ=255) tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa < 2:702561/139‑1 (MQ=255) tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa < 1:616960/139‑1 (MQ=255) aCCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg < 1:498534/139‑1 (MQ=255) aaaaGGGTGCGGAGGATGGCGACGACGGCGCAGGATCCAGCCCGGCGCGCCGAGCACCGCTGTCGGCCCCAGCGAAATCCGGATGCCCGGCAAAATTTATTTGCTGGCGCCAGCCGCGGGTGGGCTTTTGGTAAAGGta < 2:250480/139‑1 (MQ=255) tGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGaa > 2:588618/1‑139 (MQ=255) atgatgGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACcgcg < 1:310599/139‑1 (MQ=255) cgacgGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATAc > 1:736390/1‑139 (MQ=255) cgacgGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATAc > 1:4864/1‑139 (MQ=255) cccagcGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGt < 2:532009/139‑1 (MQ=255) cagcGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCg < 1:621202/139‑1 (MQ=255) cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg > 2:303220/1‑91 (MQ=255) cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg < 1:303220/91‑1 (MQ=255) cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTg > 2:632825/1‑139 (MQ=255) cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTg > 1:86248/1‑139 (MQ=255) cGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCaaa < 2:4864/139‑1 (MQ=255) gCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACcgc < 1:228880/92‑1 (MQ=255) gCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACcgc > 2:228880/1‑92 (MQ=255) aGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATAttt > 2:36981/1‑138 (MQ=255) aGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGa < 1:632825/139‑1 (MQ=255) aaTGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAAt < 1:696870/139‑1 (MQ=255) aTGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACaaa > 2:696506/1‑137 (MQ=255) aTGCCGATACCCGACAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg > 2:342560/1‑60 (MQ=255) aTGCCGATACCCGACAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg < 1:342560/60‑1 (MQ=255) tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGcc > 2:488237/1‑91 (MQ=255) tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGcc < 1:488237/91‑1 (MQ=255) tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTg > 2:160988/1‑119 (MQ=255) tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTg < 1:160988/119‑1 (MQ=255) tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAg > 2:148680/1‑139 (MQ=255) tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAg < 1:612216/139‑1 (MQ=255) ccGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCAGCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGcc < 2:728736/139‑1 (MQ=255) cGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg > 1:220533/1‑49 (MQ=255) cGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg < 2:220533/49‑1 (MQ=255) gCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCt < 2:249932/94‑1 (MQ=255) gCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCt > 1:249932/1‑94 (MQ=255) gCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGt > 2:520954/1‑139 (MQ=255) gCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGt > 1:519235/1‑139 (MQ=255) tttGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGc < 2:426187/138‑1 (MQ=255) cTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCa < 1:696506/138‑1 (MQ=255) cGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAacca > 2:506345/1‑139 (MQ=255) cGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAacca > 1:464422/1‑139 (MQ=255) ccAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccacc < 2:384530/139‑1 (MQ=255) ccAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccacc < 2:103696/139‑1 (MQ=255) aggCACCGG‑CAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAg < 2:678032/137‑1 (MQ=255) | CTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAG > NZ_CP009273/4588927‑4589184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |