Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	446,387	T→C	D227G (GAT→GGT)	cyoA ←	cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	446,387	0	T	C	100.0%	87.2 / NA	25	D227G (GAT→GGT)	cyoA	cytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/15);  total (10/15)

GTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGG  >  NZ_CP009273/446256‑446513                                                                                                                                    |                                                                                                                               gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGgtg                                                                                                                          <  2:325207/138‑1 (MQ=255) gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATGCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAGGTCAGACATGGTGTTCGGCGACTGATTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCgg                                                                                                                            <  1:300102/136‑1 (MQ=255)       cGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCaa                                                                                                                    >  1:176400/1‑138 (MQ=255)           ggAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCt                                                                                                               >  2:416312/1‑139 (MQ=255)                            ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCt                                                                                                >  1:729324/1‑137 (MQ=255)                             gTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgag                                                                                             <  2:453449/139‑1 (MQ=255)                                      gCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCggg                                                                                    <  2:176400/139‑1 (MQ=255)                                          ggCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCg                                                                                 <  1:338735/138‑1 (MQ=255)                                           gcgcGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATg                                                                                                   <  2:375897/119‑1 (MQ=255)                                           gcgcGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATg                                                                                                   >  1:375897/1‑119 (MQ=255)                                                   aGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTgg                                                                       <  1:47767/139‑1 (MQ=255)                                                         tCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCg                                                                     >  2:757418/1‑135 (MQ=255)                                                          cGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGgaga                                                                 <  1:706035/138‑1 (MQ=255)                                                                       gTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                <  1:361182/126‑1 (MQ=255)                                                                       gTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                >  2:361182/1‑126 (MQ=255)                                                                                 gtgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt                                         <  1:416312/139‑1 (MQ=255)                                                                                 gtgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt                                         >  1:325376/1‑139 (MQ=255)                                                                                         cGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATc                                 <  2:737017/139‑1 (MQ=255)                                                                                            cTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGAt                                  >  1:696550/1‑135 (MQ=255)                                                                                                   gCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAgg                        <  2:566181/138‑1 (MQ=255)                                                                                                    cTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCg                      <  1:398621/139‑1 (MQ=255)                                                                                                                     tCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCa     <  1:346607/139‑1 (MQ=255)                                                                                                                         aTGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATgg  >  1:125538/1‑138 (MQ=255)                                                                                                                            cggcgCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCa     >  2:199904/1‑132 (MQ=255)                                                                                                                            cggcgCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCa     <  1:199904/132‑1 (MQ=255)                                                                                                                                    |                                                                                                                               GTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGG  >  NZ_CP009273/446256‑446513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑