Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	478,146	A→C	F572V (TTT→GTT)	acrB ←	efflux RND transporter permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	478,146	0	A	C	100.0%	116.4 / NA	32	F572V (TTT→GTT)	acrB	efflux RND transporter permease AcrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/17);  total (15/17)

CCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCG  >  NZ_CP009273/478019‑478276                                                                                                                                |                                                                                                                                   ccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGt                                                                                                                         <  2:747653/139‑1 (MQ=255)       aCGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCAt                                                                                                                   <  2:451942/139‑1 (MQ=255)                          cGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTgg                                                                                                >  2:542957/1‑139 (MQ=255)                               ttcttttcttTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGAc                                                                                           >  2:352487/1‑139 (MQ=255)                                      ctttGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGa                                                                                     <  1:542957/138‑1 (MQ=255)                                                             aCCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACg                                                              <  1:352487/138‑1 (MQ=255)                                                              ccTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACga                                                             <  2:119565/138‑1 (MQ=255)                                                                  aTTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        <  1:436306/139‑1 (MQ=255)                                                                      aGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGa                                                     >  2:619840/1‑138 (MQ=255)                                                                           tttCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAg                                                >  1:494924/1‑138 (MQ=255)                                                                                gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAg                                          >  1:232741/1‑139 (MQ=255)                                                                                     cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                       <  2:194900/137‑1 (MQ=255)                                                                                     cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                       >  1:194900/1‑137 (MQ=255)                                                                                        tCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGcc                                                                     <  2:620526/104‑1 (MQ=255)                                                                                        tCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGcc                                                                     >  1:620526/1‑104 (MQ=255)                                                                                        tCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGcc                                  >  1:179320/1‑139 (MQ=255)                                                                                         ccTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                       <  2:486957/133‑1 (MQ=255)                                                                                         ccTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                       >  1:486957/1‑133 (MQ=255)                                                                                         ccTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCAGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGccc                                 >  1:74935/1‑139 (MQ=255)                                                                                                       gctgGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                       <  2:104098/119‑1 (MQ=255)                                                                                                       gctgGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                       >  1:104098/1‑119 (MQ=255)                                                                                                                   aCCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCGGTACTGCGCAGAATACCGCCTACGc       <  1:701749/139‑1 (MQ=255)                                                                                                                      aTGGTCATAACCACGCCCTGGTCCTCAGCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTgg    <  2:247965/139‑2 (MQ=255)                                                                                                                       tGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCa                                                                                              <  2:347281/48‑1 (MQ=255)                                                                                                                       tGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCa                                                                                              >  1:347281/1‑48 (MQ=255)                                                                                                                       tGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        >  1:77131/1‑86 (MQ=255)                                                                                                                       tGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        <  2:77131/86‑1 (MQ=255)                                                                                                                       tGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTgcgc                       >  2:567056/1‑119 (MQ=255)                                                                                                                       tGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTgcgc                       <  1:567056/119‑1 (MQ=255)                                                                                                                         gTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCg  <  2:179320/138‑1 (MQ=255)                                                                                                                             tAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        >  1:756855/1‑80 (MQ=255)                                                                                                                             tAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        <  2:756855/80‑1 (MQ=255)                                                                                                                                |                                                                                                                                   CCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCG  >  NZ_CP009273/478019‑478276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑