| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 535,507 | A→G | E302G (GAA→GGA) | allB → | allantoinase AllB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 535,507 | 0 | A | G | 92.3% | 78.3 / ‑0.1 | 26 | E302G (GAA→GGA) | allB | allantoinase AllB |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (13/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TCAGGACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTT > NZ_CP009273/535379‑535639 | tCAGGACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGtt > 1:651652/1‑138 (MQ=255) aGGACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTaa > 2:590774/1‑139 (MQ=255) gACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACg < 1:590774/139‑1 (MQ=255) cTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAAt < 1:251229/139‑1 (MQ=255) aaTCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTg > 1:595144/1‑139 (MQ=255) cTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCa < 1:739439/138‑1 (MQ=255) cGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCccgccg > 2:66122/1‑139 (MQ=255) aTCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCgg > 2:50054/1‑138 (MQ=255) aGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCa > 2:614062/1‑118 (MQ=255) aGTTCGAAAAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCa < 1:614062/118‑1 (MQ=255) aaTCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGt < 1:66122/139‑1 (MQ=255) tGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGa > 2:186013/1‑138 (MQ=255) gCGAAGTTTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGgaa < 1:521983/55‑1 (MQ=255) gCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGgaa > 2:521983/1‑55 (MQ=255) ttCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGAc > 1:252749/1‑85 (MQ=255) ttCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGAc < 2:252749/85‑1 (MQ=255) cgATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGGCTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGc < 2:443504/139‑1 (MQ=255) aTCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTcc < 1:516559/72‑1 (MQ=255) aTCCGCGATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTcc > 2:516559/1‑72 (MQ=255) cgATCTGGAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTg > 2:567618/1‑139 (MQ=255) gAAAACCAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAg < 1:50054/137‑1 (MQ=255) cAGAAAGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATg > 1:602170/1‑138 (MQ=255) aaaGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAg < 1:635416/128‑1 (MQ=255) aaaGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAg > 2:635416/1‑128 (MQ=255) aaaGGCATGTGGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACg < 2:651652/139‑1 (MQ=255) tgtgGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGtt > 1:427764/1‑139 (MQ=255) tgGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAAcat > 2:340555/1‑83 (MQ=255) tgGGGAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAAcat < 1:340555/83‑1 (MQ=255) | TCAGGACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTT > NZ_CP009273/535379‑535639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |