| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 557,194 | A→G | Q681R (CAG→CGG) | sfmD → | fimbrial biogenesis usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 557,194 | 0 | A | G | 97.6% | 133.9 / ‑3.8 | 41 | Q681R (CAG→CGG) | sfmD | fimbrial biogenesis usher protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (23/17); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.59e-01 | |||||||||||
CACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCA > NZ_CP009273/557067‑557308 | cACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCg > 2:691071/1‑139 (MQ=255) gCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGt < 2:528103/139‑1 (MQ=255) gggTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCggg < 2:502550/139‑1 (MQ=255) aTACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATg > 1:537553/1‑138 (MQ=255) tACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGa > 1:415682/1‑138 (MQ=255) aCCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTc > 1:503487/1‑129 (MQ=255) aCCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTc < 2:503487/129‑1 (MQ=255) acGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGagcc < 2:138919/139‑1 (MQ=255) acGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGagcc < 2:537553/139‑1 (MQ=255) cAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCt > 2:481921/1‑139 (MQ=255) gcgcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGaaaa < 2:549209/115‑1 (MQ=255) gcgcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGaaaa > 1:549209/1‑115 (MQ=255) gcgcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGagcc > 1:141452/1‑132 (MQ=255) gcgcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGagcc < 2:141452/132‑1 (MQ=255) gcgcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCtt > 1:374433/1‑138 (MQ=255) ccACGGCAAACTGGCAGGCCGCAAACGGTACGATGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGATAACTGGCGGCTGTCTGGCGGGGCGGTCGGGCATGAAAAAGGCATAACGCTGAGCCAGCCTTTAgg > 1:664076/1‑139 (MQ=255) cGGCAAACTGGCAGGCCGCATACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCGGGCTGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAgggta < 2:150327/139‑3 (MQ=255) gCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCa > 2:699562/1‑104 (MQ=255) gCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCa < 1:699562/104‑1 (MQ=255) gCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGa < 1:149927/139‑1 (MQ=255) cTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGt > 1:608276/1‑130 (MQ=255) cTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGt < 2:608276/130‑1 (MQ=255) aCGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTaaa < 2:397612/137‑1 (MQ=255) aCGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTg > 1:257500/1‑139 (MQ=255) gCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGt > 2:261175/1‑69 (MQ=255) gCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGt < 1:261175/69‑1 (MQ=255) cTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGACGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATGAAAGCGCCTGgcg < 2:415682/139‑1 (MQ=255) gggggCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGgcgc < 1:481921/137‑1 (MQ=255) gggggCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGgcgc < 1:290086/137‑1 (MQ=255) ggCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCg > 1:642169/1‑139 (MQ=255) ggTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGgtg > 1:594705/1‑138 (MQ=255) cGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGa > 1:419759/1‑138 (MQ=255) aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 2:746115/1‑139 (MQ=255) aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 1:244238/1‑139 (MQ=255) aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 2:626179/1‑139 (MQ=255) aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 2:509865/1‑139 (MQ=255) aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 2:504848/1‑139 (MQ=255) cgATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCa > 2:615555/1‑138 (MQ=255) cgATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCa > 2:400672/1‑138 (MQ=255) aCGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGgcgc > 1:202583/1‑103 (MQ=255) aCGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGgcgc < 2:202583/103‑1 (MQ=255) | CACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCA > NZ_CP009273/557067‑557308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |