Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 37,524 T→G S101R (AGC→CGC)  caiC ← crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927337,5240TG100.0% 127.0 / NA 35S101R (AGC→CGC) caiCcrotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/14);  total (21/14)

GGAAGTGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCA  >  NZ_CP009273/37391‑37655
                                                                                                                                     |                                                                                                                                   
ggAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCt                                                                                                                                <  2:279227/139‑1 (MQ=255)
    gcgCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGca                                                                                                                            >  2:665505/3‑139 (MQ=255)
                tCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAggcg                                                                                                                   <  2:353385/136‑1 (MQ=255)
                  aGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGt                                                                                                              >  1:432160/1‑139 (MQ=255)
                       aaTGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         <  1:665505/139‑1 (MQ=255)
                       aaTGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         >  1:649835/1‑139 (MQ=255)
                          gtgCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTTATCgg                                                                                                       >  2:260697/1‑138 (MQ=255)
                                           cATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAAt                                                                                     >  2:714230/1‑139 (MQ=255)
                                                          tCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCgg                                                                                                       <  1:324912/106‑1 (MQ=255)
                                                          tCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCgg                                                                                                       >  2:324912/1‑106 (MQ=255)
                                                                  tACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCaa                                                               >  1:238403/1‑138 (MQ=255)
                                                                      tAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGAt                                                          <  2:432160/139‑1 (MQ=255)
                                                                        ggATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGc                                                                               >  1:337554/1‑116 (MQ=255)
                                                                        ggATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGc                                                                               <  2:337554/116‑1 (MQ=255)
                                                                           tAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAAtt                                                     >  1:487230/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCt                                                                                                                                <  2:523819/56‑1 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCt                                                                                                                                >  1:523819/1‑56 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAggc                                                                                                                    >  1:253372/1‑68 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAggc                                                                                                                    <  2:253372/68‑1 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  2:710105/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             <  1:714230/139‑1 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  1:135501/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  1:516786/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTA‑TTTCCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGt                                            >  1:435041/1‑139 (MQ=255)
                                                                                          gTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTc                                        >  2:144118/1‑137 (MQ=255)
                                                                                           tCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCgag                                     <  1:144118/139‑1 (MQ=255)
                                                                                                  cagGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGt                              >  1:512133/1‑139 (MQ=255)
                                                                                                       aCGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAAc                         >  2:278303/1‑139 (MQ=255)
                                                                                                         gCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTg                             >  1:713150/1‑133 (MQ=255)
                                                                                                         gCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTg                             <  2:713150/133‑1 (MQ=255)
                                                                                                         gCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCt                       >  2:620352/1‑139 (MQ=255)
                                                                                                          ccTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCtt                      >  2:397643/1‑139 (MQ=255)
                                                                                                                       cAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCg          <  1:588131/138‑1 (MQ=255)
                                                                                                                       cAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCg          <  2:541987/138‑1 (MQ=255)
                                                                                                                              cACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCa  <  1:669932/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GGAAGTGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCA  >  NZ_CP009273/37391‑37655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑