Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	659,797	A→C	S284A (TCG→GCG)	rlpA ←	endolytic peptidoglycan transglycosylase RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	659,797	0	A	C	100.0%	96.1 / NA	27	S284A (TCG→GCG)	rlpA	endolytic peptidoglycan transglycosylase RlpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/12);  total (15/12)

CCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTG  >  NZ_CP009273/659668‑659919                                                                                                                                  |                                                                                                                           ccGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTg                                                                                                                   <  2:462612/139‑1 (MQ=255)     gcCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGgt                                                                                                               >  1:193413/1‑139 (MQ=255)                   cgcgACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAgg                                                                                                 >  2:73882/1‑139 (MQ=255)                   cgcgACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAgg                                                                                                 >  2:391025/1‑139 (MQ=255)                           gggACGCCGAACTTCTGTCCCAGTCGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTg                                                                                         >  2:469211/1‑139 (MQ=255)                                  cGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGt                                                                                  >  2:564272/1‑139 (MQ=255)                                         cTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGa                                                                            <  1:564272/138‑1 (MQ=255)                                         cTGTCCCAGTCGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGa                                                                            <  1:469211/138‑1 (MQ=255)                                            tCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACgg                                                                         <  2:476484/138‑1 (MQ=255)                                            tCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACgg                                                                         <  2:366089/138‑1 (MQ=255)                                                                  tGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAgg                                                                                                 >  2:85347/1‑92 (MQ=255)                                                                  tGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAgg                                                                                                 <  1:85347/92‑1 (MQ=255)                                                                  tGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGgcg                                                  >  2:268405/1‑139 (MQ=255)                                                                  tGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGgcg                                                  >  1:763611/1‑139 (MQ=255)                                                                      cACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAgg                                              >  2:361738/1‑139 (MQ=255)                                                                           gCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCg                                         >  1:413999/1‑139 (MQ=255)                                                                                aTCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCg                                    <  1:268405/139‑1 (MQ=255)                                                                                   gCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGTCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCTCAGGCGTCGGTTCGCTg                                 <  1:79161/139‑1 (MQ=255)                                                                                     ttACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGcc                               >  1:67263/1‑139 (MQ=255)                                                                                          gCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGcc                               <  1:565479/134‑1 (MQ=255)                                                                                          gCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGcc                               >  2:565479/1‑134 (MQ=255)                                                                                          gCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCa                          <  1:616640/139‑1 (MQ=255)                                                                                                    gCACCATAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCa                  <  2:350236/137‑1 (MQ=255)                                                                                                          tAAAGTTGCCGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCt            >  1:693969/1‑137 (MQ=255)                                                                                                                  ccGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGa                                                                            <  1:757684/65‑1 (MQ=255)                                                                                                                  ccGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGa                                                                            >  2:757684/1‑65 (MQ=255)                                                                                                                  ccGCTGGCGCTTTGCGCGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTg  >  2:585272/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                           CCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTG  >  NZ_CP009273/659668‑659919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑