Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	771,407	A→G	T35A (ACA→GCA)	tolR →	colicin uptake protein TolR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	771,407	0	A	G	94.4%	54.5 / ‑3.0	18	T35A (ACA→GCA)	tolR	colicin uptake protein TolR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (8/9);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTACCGTTAGCGAGAGCAACAAGGGGTAAGCCATGGCCAGAGCGCGTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGACAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTA  >  NZ_CP009273/771271‑771515                                                                                                                                         |                                                                                                             gTTTACCGTTAGCGAGAGCAACAAGGGGTAAGCCATGGCCAGAGCGCGTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCa                                                                                                            <  2:100217/139‑1 (MQ=255)      ccGTTAGCGAGAGCAACAAGGGGTAAGCCATGGCCAGAGCGCGTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGcc                                                                                                       <  2:451481/139‑1 (MQ=255)                              aaGCCATGGCCAGAGCGCGTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGt                                                                               >  2:401819/1‑139 (MQ=255)                                     ggCCAGAGCGCGTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTg                                                                        >  2:317932/1‑139 (MQ=255)                                                gTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTAc                                                             >  1:339200/1‑139 (MQ=255)                                                     cGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCAcc                                                                                             >  1:291130/1‑102 (MQ=255)                                                     cGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCAcc                                                                                             <  2:291130/102‑1 (MQ=255)                                                                                       ttGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGt                      <  1:46554/139‑1 (MQ=255)                                                                                               ttGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCCGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGGGGAGGAACGAACATACGCCCGTGAttgttt              >  2:238037/1‑138 (MQ=255)                                                                                               ttGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGcc                                                                                                       <  2:502769/50‑1 (MQ=255)                                                                                               ttGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGcc                                                                                                       >  1:502769/1‑50 (MQ=255)                                                                                               ttGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAAAGATAATCCGCCAGTGAttgtt               >  1:187272/1‑138 (MQ=255)                                                                                                  cTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGaa            <  1:317932/138‑1 (MQ=255)                                                                                                  cTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGaa            <  1:191884/138‑1 (MQ=255)                                                                                                         tACTGCTGGTGCTGTTGCTGATCTTTATGGCGACAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTgg    >  1:318175/1‑139 (MQ=255)                                                                                                          aCTGCTGGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGGGAGGAGGAACGATAATCCGCCAGTGATTGTTGGAGTGTCTgg    >  2:191884/1‑138 (MQ=255)                                                                                                           ctgctgGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTa  <  2:187272/139‑1 (MQ=255)                                                                                                           ctgctgGTGCTGTTGCTGATCTTTATGGCGGCAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTa  <  1:401819/139‑1 (MQ=255)                                                                                                                                         |                                                                                                             GTTTACCGTTAGCGAGAGCAACAAGGGGTAAGCCATGGCCAGAGCGCGTGGACGAGGTCGTCGCGATCTCAAGTCCGAAATCAACATTGTACCGTTGCTGGACGTACTGCTGGTGCTGTTGCTGATCTTTATGGCGACAGCGCCCATCATCACCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTA  >  NZ_CP009273/771271‑771515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑