Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,032,221	T→C	F6S (TTC→TCC)	hyaF →	hydrogenase expression/formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,032,221	0	T	C	100.0%	40.6 / NA	13	F6S (TTC→TCC)	hyaF	hydrogenase expression/formation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/6);  total (7/6)

CGGAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACCGAGTATGGCGGCC  >  NZ_CP009273/1032109‑1032321                                                                                                                 |                                                                                                     cggAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATctgct                                                                                             >  1:748992/1‑122 (MQ=255) cggAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATctgct                                                                                             <  2:748992/122‑1 (MQ=255)                                 aCCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCTACCGAACGATGACAGTTTCAGCATGAATCCAc                                              <  1:675991/137‑1 (MQ=255)                                 aCCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCAc                                              >  2:675991/1‑137 (MQ=255)                                             aaCTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCAt                                                      >  1:522512/1‑117 (MQ=255)                                             aaCTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCAt                                                      <  2:522512/117‑1 (MQ=255)                                                          gATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAatgat                    >  2:871/1‑138 (MQ=255)                                                            tGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGt                                >  1:443665/1‑124 (MQ=255)                                                            tGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGt                                <  2:443665/124‑1 (MQ=255)                                                            tGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGaa                 >  1:523316/1‑139 (MQ=255)                                                                           aaCCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACCGAGTATGGCGGcc  <  2:307112/139‑1 (MQ=255)                                                                             ccGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATctgct                                                                                             <  1:323524/46‑1 (MQ=255)                                                                             ccGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTCCCATctgct                                                                                             >  2:323524/1‑46 (MQ=255)                                                                                                                 |                                                                                                     CGGAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACCGAGTATGGCGGCC  >  NZ_CP009273/1032109‑1032321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑