Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,035,547	A→G	I266V (ATC→GTC)	appB →	cytochrome d ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,035,547	0	A	G	100.0%	112.0 / NA	31	I266V (ATC→GTC)	appB	cytochrome d ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

TGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACG  >  NZ_CP009273/1035418‑1035675                                                                                                                                  |                                                                                                                                 tgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCgc                                                                                                                         >  1:363949/1‑139 (MQ=255)  gggTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCgct                                                                                                                        >  2:16838/1‑139 (MQ=255)         tATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGa                                                                                                                 <  1:25068/139‑1 (MQ=255)                     tGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGcc                                                                                                       <  2:266427/137‑1 (MQ=255)                      gTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCa                                                                                                     <  2:363949/138‑1 (MQ=255)                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGa                                                                                              >  2:756383/1‑139 (MQ=255)                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGa                                                                                              <  1:388180/139‑1 (MQ=255)                                    ggATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATg                                                                                      <  1:193733/139‑1 (MQ=255)                                         cTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGAt                                                                                 >  2:113602/1‑139 (MQ=255)                                               gTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTg                                                                                                  >  2:13649/1‑116 (MQ=255)                                               gTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTg                                                                                                  <  1:13649/116‑1 (MQ=255)                                                                          tggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGAt                                                >  2:222838/1‑139 (MQ=255)                                                                             cAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGc                                             <  1:756383/139‑1 (MQ=255)                                                                                 gCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATg                                                                                      <  1:50360/94‑1 (MQ=255)                                                                                 gCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATg                                                                                      >  2:50360/1‑94 (MQ=255)                                                                                          tGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGccc                                                                                                      >  1:23609/1‑69 (MQ=255)                                                                                          tGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGccc                                                                                                      <  2:23609/69‑1 (MQ=255)                                                                                          tGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCaa                                >  1:120000/1‑139 (MQ=255)                                                                                            ccTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAAtt                              <  1:590684/139‑1 (MQ=255)                                                                                               ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTCTCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTgg                           >  2:345425/1‑139 (MQ=255)                                                                                               ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTgg                           >  1:346595/1‑139 (MQ=255)                                                                                               ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTgg                           >  2:676491/1‑139 (MQ=255)                                                                                               ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTgg                           >  2:320292/1‑139 (MQ=255)                                                                                               ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTgg                           >  2:435688/1‑139 (MQ=255)                                                                                                 aTGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCg                         >  1:484826/1‑139 (MQ=255)                                                                                                    aataatTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGa                      >  1:546463/1‑139 (MQ=255)                                                                                                          tttGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCa                >  2:66939/1‑139 (MQ=255)                                                                                                              tCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACggc            <  2:546463/139‑1 (MQ=255)                                                                                                                      cccGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCa    <  2:346595/139‑1 (MQ=255)                                                                                                                      cccGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCa    <  1:113602/139‑1 (MQ=255)                                                                                                                        cGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACg  <  1:222838/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 TGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACG  >  NZ_CP009273/1035418‑1035675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑