Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,060,660	T→C	L390P (CTC→CCC)	yccE →	YccE family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,060,660	0	T	C	100.0%	62.2 / NA	19	L390P (CTC→CCC)	yccE	YccE family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/7);  total (12/7)

CTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTT  >  NZ_CP009273/1060538‑1060793                                                                                                                           |                                                                                                                                      cTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACttttt                                                                                                                       >  1:485651/1‑139 (MQ=255)     ttatttatTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTc                                                                                                                   <  2:470881/139‑1 (MQ=255)       atttattGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGTAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAg                                                                                                                 >  2:611679/1‑139 (MQ=255)           attGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAccccc                                                                                                                                     <  2:590390/115‑1 (MQ=255)           attGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAccccc                                                                                                                                     >  1:590390/1‑115 (MQ=255)                         tgtgGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACaa                                                                                                >  1:462895/1‑138 (MQ=255)                             gCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAg                                                                                            >  2:363300/1‑138 (MQ=255)                              cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAgg                                                                                           >  1:30228/1‑138 (MQ=255)                                        cTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAaatgaatg                                                                                  <  1:450609/137‑1 (MQ=255)                                                          ttCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTaaa                                                               <  1:611679/138‑1 (MQ=255)                                                                    gAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTc                                                                                                                   >  2:263168/1‑76 (MQ=255)                                                                    gAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTc                                                                                                                   <  1:263168/76‑1 (MQ=255)                                                                                     ttATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCat                                   <  1:114417/139‑1 (MQ=255)                                                                                        tttAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATg                                >  2:660764/1‑139 (MQ=255)                                                                                                 cacTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCt                                                                                                                    <  1:491635/46‑1 (MQ=255)                                                                                                 cacTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCt                                                                                                                    >  2:491635/1‑46 (MQ=255)                                                                                                         aTCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGttat               >  2:501529/1‑139 (MQ=255)                                                                                                              tATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATg                                                       <  2:68814/94‑1 (MQ=255)                                                                                                              tATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATg                                                       >  1:68814/1‑94 (MQ=255)                                                                                                              tATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtc          >  2:662133/1‑139 (MQ=255)                                                                                                                      ttACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtt  >  1:333012/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                      CTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTT  >  NZ_CP009273/1060538‑1060793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑