| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,143,854 | A→G | K260E (AAA→GAA) | plsX → | phosphate acyltransferase PlsX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,143,854 | 0 | A | G | 91.7% | 69.7 / ‑0.1 | 24 | K260E (AAA→GAA) | plsX | phosphate acyltransferase PlsX |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (7/15); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AGCCAATGAGTTGTTAACTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTC > NZ_CP009273/1143724‑1143989 | aGCCAATGAGTTGTTAACTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACgg > 2:568356/1‑139 (MQ=255) aaTGAGTTGTTAACTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCgt < 2:211831/139‑1 (MQ=255) ttAACTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTa < 1:568356/138‑1 (MQ=255) aCTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTg < 1:308878/138‑1 (MQ=255) aCTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTg < 1:754236/138‑1 (MQ=255) gCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGttat > 2:157952/1‑138 (MQ=255) gCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGttat > 2:362463/1‑138 (MQ=255) gATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGtt < 1:561420/139‑1 (MQ=255) ggAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGAt < 2:486289/139‑1 (MQ=255) aaTGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGtggtgg < 1:166628/90‑1 (MQ=255) aaTGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGtggtgg > 2:166628/1‑90 (MQ=255) tGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGt < 1:157952/139‑1 (MQ=255) caTTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCt < 1:187747/139‑1 (MQ=255) gATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGAc < 1:362463/139‑1 (MQ=255) aTGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTAc > 2:623374/1‑76 (MQ=255) aTGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTAc < 1:623374/76‑1 (MQ=255) ttgtCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACgg > 1:549186/1‑139 (MQ=255) aTGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACaa > 2:451010/1‑81 (MQ=255) aTGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACaa < 1:451010/81‑1 (MQ=255) tctcTGCTGAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGa < 1:246107/138‑1 (MQ=255) tgctgAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGAtt < 1:608554/136‑1 (MQ=255) ctgAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGAc < 2:404943/105‑1 (MQ=255) ctgAAATCTCAGGGTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGAc > 1:404943/1‑105 (MQ=255) gTGAAGGGGAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATaaa < 2:268263/139‑1 (MQ=255) ggggAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTc < 2:169892/139‑1 (MQ=255) | AGCCAATGAGTTGTTAACTGGCAAGACAGATGTGCTGGTTTGTGACGGCTTTACAGGAAATGTCACATTAAAGACGATGGAAGGTGTTGTCAGGATGTTCCTTTCTCTGCTGAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTC > NZ_CP009273/1143724‑1143989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |