Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,276,082	T→G	S255A (TCA→GCA)	narG →	nitrate reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,276,082	0	T	G	100.0%	82.4 / NA	23	S255A (TCA→GCA)	narG	nitrate reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/10);  total (13/10)

ATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAA  >  NZ_CP009273/1275947‑1276216                                                                                                                                        |                                                                                                                                       aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCa                                                                                                                                      >  1:85050/1‑138 (MQ=255)      cGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTg                                                                                                                                <  2:100827/139‑1 (MQ=255)                                 gTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAc                                                                                                     >  1:443797/1‑139 (MQ=255)                                     tGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtct                                                                                                 >  2:98417/1‑139 (MQ=255)                                            tGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTg                                                                                           >  1:367032/1‑138 (MQ=255)                                            tGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCAGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTg                                                                                           >  1:48228/1‑138 (MQ=255)                                                                          aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCt                                                                                                        >  2:345349/1‑95 (MQ=255)                                                                          aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCt                                                                                                        <  1:345349/95‑1 (MQ=255)                                                                          aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc                                                            >  1:492073/1‑139 (MQ=255)                                                                          aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc                                                            >  1:85807/1‑139 (MQ=255)                                                                             aaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt                                                         <  2:85807/139‑1 (MQ=255)                                                                                        ccGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCt                                                                                                        >  1:425086/1‑81 (MQ=255)                                                                                        ccGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCt                                                                                                        <  2:425086/81‑1 (MQ=255)                                                                                         cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCg                                                          <  1:236127/126‑1 (MQ=255)                                                                                         cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCg                                                          >  2:236127/1‑126 (MQ=255)                                                                                                  ctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGa                                       <  2:443797/136‑1 (MQ=255)                                                                                                          tACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa                             >  1:567733/1‑138 (MQ=255)                                                                                                          tACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa                             <  2:567733/138‑1 (MQ=255)                                                                                                                    gCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGAttt                  <  2:367032/139‑3 (MQ=255)                                                                                                                    gCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCt                  >  2:508338/1‑139 (MQ=255)                                                                                                                    gCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCt                  >  2:76871/1‑139 (MQ=255)                                                                                                                          tcatcGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggct            <  1:198389/139‑1 (MQ=255)                                                                                                                                    ggggGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa  <  1:98417/139‑1 (MQ=255)                                                                                                                                     ggggCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa  >  2:557739/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                       ATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAA  >  NZ_CP009273/1275947‑1276216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑