| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,433,342 | A→G | F567S (TTC→TCC) | nifJ ← | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,433,342 | 0 | A | G | 97.1% | 124.3 / ‑3.3 | 34 | F567S (TTC→TCC) | nifJ | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (14/19); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
ATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGAAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACG > NZ_CP009273/1433211‑1433449 | aTTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGc > 2:278212/1‑139 (MQ=255) aCGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCAt < 1:230391/138‑1 (MQ=255) aCGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGAAAAAAGCCAt < 2:331804/138‑1 (MQ=255) agcgccagAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGa < 2:715855/139‑1 (MQ=255) agcgccagAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGa < 1:231643/139‑1 (MQ=255) agcgccagAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGa < 2:426241/139‑1 (MQ=255) agcgccagAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATGCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGa < 2:700799/139‑1 (MQ=255) gagCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTAt > 1:596914/1‑138 (MQ=255) gCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTaa > 2:177921/1‑139 (MQ=255) tGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGcc < 2:250645/117‑1 (MQ=255) tGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGcc > 1:250645/1‑117 (MQ=255) gCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGa > 2:656770/1‑119 (MQ=255) gCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGa < 1:656770/119‑1 (MQ=255) ttCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACg > 2:153173/1‑129 (MQ=255) ttCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACg < 1:153173/129‑1 (MQ=255) tGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCggg > 2:135815/1‑139 (MQ=255) ttGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGAttt > 2:366312/1‑139 (MQ=255) tGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGAc < 1:539873/97‑1 (MQ=255) tGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGAc > 2:539873/1‑97 (MQ=255) cTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGgccg < 2:127971/109‑1 (MQ=255) cTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGgccg > 1:127971/1‑109 (MQ=255) gTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGcc > 1:670772/1‑79 (MQ=255) gTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGcc < 2:670772/79‑1 (MQ=255) aCTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGtt < 1:177921/138‑1 (MQ=255) cccTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAgcgcg < 2:596914/139‑1 (MQ=255) cAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCtttt > 1:657591/1‑139 (MQ=255) tCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAgg < 1:402073/84‑1 (MQ=255) tCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAgg > 2:402073/1‑84 (MQ=255) ggCGCTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAac < 1:135815/139‑1 (MQ=255) gcgcTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGt < 1:713932/103‑1 (MQ=255) gcgcTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGt > 2:713932/1‑103 (MQ=255) gcgcTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAaca < 1:366312/139‑1 (MQ=255) cgcTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAacac < 1:179471/139‑1 (MQ=255) gcTATCGCCAGGCAGAATTTGCGTCAGATGGGAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACg < 1:228485/139‑1 (MQ=255) | ATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATTCTGCGAGGGCGCTATCGCCAGGCAGAATTTGCGTCAGATGGAAAAAAGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACG > NZ_CP009273/1433211‑1433449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |