Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,444,183	T→G	Q475P (CAG→CCG)	tynA ←	primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,444,183	0	T	G	100.0%	212.7 / NA	58	Q475P (CAG→CCG)	tynA	primary‑amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/28);  total (30/28)

ATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACGGGGCATCT  >  NZ_CP009273/1444051‑1444317                                                                                                                                     |                                                                                                                                       aTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCa                                                                                                                                  <  2:480293/139‑1 (MQ=255)  tGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCAt                                                                                                                                 >  1:51908/1‑139 (MQ=255)       gTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                                            <  1:598267/139‑1 (MQ=255)       gTAGCACCGGAAGCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTCCTGACGTTGGGCGGGCCCATTTCCt                                                                                                                            <  2:374077/139‑1 (MQ=255)          gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          <  1:14927/138‑1 (MQ=255)          gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          <  1:185552/138‑1 (MQ=255)                gCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTta                                                                                                                    >  2:31936/1‑138 (MQ=255)                               gTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCAt                                                                                                    <  1:522130/139‑1 (MQ=255)                                  ccGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAc                                                                                                 >  1:179763/1‑139 (MQ=255)                                   cGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACg                                                                                                >  2:322735/1‑139 (MQ=255)                                        tCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCa                                                                                            <  1:596715/138‑1 (MQ=255)                                               aGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTccgg                                                                                                            <  2:516513/115‑1 (MQ=255)                                               aGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTccgg                                                                                                            >  1:516513/1‑115 (MQ=255)                                                      gTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGAt                                                                                                                         <  2:104422/95‑1 (MQ=255)                                                      gTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGAt                                                                                                                         >  1:104422/1‑95 (MQ=255)                                                      gTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTc                                                                                                               <  1:412881/105‑1 (MQ=255)                                                      gTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTc                                                                                                               >  2:412881/1‑105 (MQ=255)                                                      gTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAgcg                                                                             <  1:31936/139‑1 (MQ=255)                                                                     aTAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                                            >  2:477760/1‑77 (MQ=255)                                                                     aTAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                                            <  1:477760/77‑1 (MQ=255)                                                                     aTAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACg                                                                                                >  2:191037/1‑105 (MQ=255)                                                                     aTAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACg                                                                                                <  1:191037/105‑1 (MQ=255)                                                                          tACCCACTGTACTGATCCAGCGCACCGCTAGCTCCCGGCGTTCGATACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATGCTCCGGCCCGGCATAACGTTCAAATCCCGCGATAGCGCGGGGGATCTCCATCGGCa                                                          <  2:383246/138‑1 (MQ=255)                                                                                 tGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          >  1:396466/1‑67 (MQ=255)                                                                                 tGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          <  2:396466/67‑1 (MQ=255)                                                                                 tGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAgtg                                                  >  2:482514/1‑139 (MQ=255)                                                                                 tGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAgtg                                                  >  1:439571/1‑139 (MQ=255)                                                                                   tACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAgtg                                                  >  2:285004/1‑137 (MQ=255)                                                                                        aTCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCgg                                           >  1:574448/1‑139 (MQ=255)                                                                                        aTCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCgg                                           >  1:528564/1‑139 (MQ=255)                                                                                        aTCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCgg                                           >  1:520711/1‑139 (MQ=255)                                                                                           cAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATaa                                                                                                  >  2:345363/1‑81 (MQ=255)                                                                                           cAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATaa                                                                                                  <  1:345363/81‑1 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGc                                                                                                                       >  1:274941/1‑58 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGc                                                                                                                       <  2:274941/58‑1 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGa                                                                          <  1:241023/103‑1 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGa                                                                          >  2:241023/1‑103 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATg                                      >  2:45576/1‑139 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATg                                      >  1:76301/1‑139 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATg                                      >  1:466561/1‑139 (MQ=255)                                                                                             gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATg                                      >  1:385450/1‑139 (MQ=255)                                                                                             gcgcACCAATAACTCCCGGCGTTCGGTACTGTCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCGCGCCAGTGCAGTCGGCGATg                                      >  2:689173/1‑139 (MQ=255)                                                                                              cgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATgg                                     <  2:385450/139‑1 (MQ=255)                                                                                                   cacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCa                                <  2:520711/139‑1 (MQ=255)                                                                                                           cccGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAg                        >  2:746250/1‑139 (MQ=255)                                                                                                             cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATa                                                                                        >  1:732067/1‑73 (MQ=255)                                                                                                             cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATa                                                                                        <  2:732067/73‑1 (MQ=255)                                                                                                             cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa                      <  1:545489/139‑1 (MQ=255)                                                                                                               gCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa                      <  1:692179/137‑1 (MQ=255)                                                                                                               gCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa                      >  2:692179/1‑137 (MQ=255)                                                                                                               gCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACt                    >  1:253664/1‑139 (MQ=255)                                                                                                                    cGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa                      >  1:703983/1‑132 (MQ=255)                                                                                                                    cGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa                      <  2:703983/132‑1 (MQ=255)                                                                                                                       tACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGa            <  1:285297/139‑1 (MQ=255)                                                                                                                            aCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCgg                                                            >  2:420923/1‑86 (MQ=255)                                                                                                                            aCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCgg                                                            <  1:420923/86‑1 (MQ=255)                                                                                                                            aCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg       <  2:140991/139‑1 (MQ=255)                                                                                                                            aCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgg         <  1:18657/137‑1 (MQ=255)                                                                                                                                 gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg       <  2:689623/134‑1 (MQ=255)                                                                                                                                 gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg       >  1:689623/1‑134 (MQ=255)                                                                                                                                 gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACGGGGCATCt  >  2:761849/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       ATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACGGGGCATCT  >  NZ_CP009273/1444051‑1444317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑