Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	143.4 / NA	40	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/20);  total (20/20)

TACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGG  >  NZ_CP009273/49783‑50044                                                                                                                                |                                                                                                                                       tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACg                                                                                                                              >  1:687603/1‑138 (MQ=255)  aCGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGc                                                                                                                             >  1:647483/1‑138 (MQ=255)  aCGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGc                                                                                                                             >  2:729227/1‑138 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                                                                                           <  1:262431/139‑1 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                            <  2:399792/138‑1 (MQ=255)       taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACAcc                                                                                                                       <  1:228543/139‑1 (MQ=255)        aGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                                                                                      >  2:643780/1‑139 (MQ=255)         gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                                                                                      >  1:52635/1‑138 (MQ=255)             cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                                  >  2:378940/1‑138 (MQ=255)               aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaata                                                                                                                >  2:258810/1‑138 (MQ=255)               aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaata                                                                                                                >  1:539466/1‑138 (MQ=255)                              gggAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                                                                 <  2:8877/138‑1 (MQ=255)                                        aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCGCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAcc                                                                                      <  1:258810/139‑1 (MQ=255)                                            aTCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                                                                                  <  2:50306/139‑1 (MQ=255)                                                    gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                                                                          >  2:201567/1‑139 (MQ=255)                                                        gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                                                                       <  1:201567/138‑1 (MQ=255)                                                        gcggcgTTAGCGGTAGATCGCGTTAACGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                                                                       <  1:323498/138‑1 (MQ=255)                                                           gcgTTAGTGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                                   >  2:638454/1‑139 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAcc                                                                                      <  1:31992/120‑1 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAcc                                                                                      >  2:31992/1‑120 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                                                                     >  1:298678/1‑121 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                                                                     <  2:298678/121‑1 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                                   >  1:330170/1‑139 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                                   >  1:565745/1‑139 (MQ=255)                                                                       gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGa                                                        <  2:330170/138‑1 (MQ=255)                                                                           gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTCCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                                   <  1:166710/139‑1 (MQ=255)                                                                           gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                                   <  2:261606/139‑1 (MQ=255)                                                                           gcgTTATCGGCATCGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                                   <  2:403206/139‑1 (MQ=255)                                                                               tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                                                                                           <  2:27518/63‑1 (MQ=255)                                                                               tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                                                                                           >  1:27518/1‑63 (MQ=255)                                                                                          aaaaCGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGTCAGGACGCAAAAATATTATCCTcag                                    <  1:554657/139‑1 (MQ=255)                                                                                               gCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGt                                >  1:555146/1‑138 (MQ=255)                                                                                                   tGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAACAATATTATCCTCAGCAGTCAAcc                           >  2:89733/1‑139 (MQ=255)                                                                                                   tGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAAcc                           <  1:378940/139‑1 (MQ=255)                                                                                                               tgcctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGa               >  2:452514/1‑139 (MQ=255)                                                                                                                 cctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGa               <  2:539466/137‑1 (MQ=255)                                                                                                                     ccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGt         <  1:638454/139‑1 (MQ=255)                                                                                                                        aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAAc      >  1:172570/1‑139 (MQ=255)                                                                                                                           tcGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTg   >  1:213795/1‑139 (MQ=255)                                                                                                                             gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTgg  <  1:643780/138‑1 (MQ=255)                                                                                                                                |                                                                                                                                       TACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGG  >  NZ_CP009273/49783‑50044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑