Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,551,828	T→G	A156A (GCA→GCC)	ddpF ←	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,551,828	0	T	G	100.0%	131.2 / NA	37	A156A (GCA→GCC)	ddpF	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/21);  total (16/21)

TGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGTGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCACTACTACG  >  NZ_CP009273/1551692‑1551962                                                                                                                                         |                                                                                                                                       tgtgtgAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGc                                                                                                                                      >  1:341726/1‑139 (MQ=255)             gCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGc                                                                                                                          >  1:551289/1‑139 (MQ=255)                        aGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGcc                                                                                                               >  2:122186/1‑139 (MQ=255)                        aGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGcc                                                                                                               >  2:382839/1‑139 (MQ=255)                        aGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGcc                                                                                                               >  1:644540/1‑139 (MQ=255)                           ccGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCt                                                                                                            <  1:382839/139‑1 (MQ=255)                                         tAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGgaga                                                                                                <  1:256284/137‑1 (MQ=255)                                         tAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAAc                                                                                              >  1:452163/1‑139 (MQ=255)                                            cGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCaa                                                                                                                              >  2:324688/1‑104 (MQ=255)                                            cGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCaa                                                                                                                              <  1:324688/104‑1 (MQ=255)                                                        aTTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCgg                                                                               <  1:154912/139‑1 (MQ=255)                                                             ggATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCgag                                                                          <  1:475867/139‑1 (MQ=255)                                                                          aaCGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACg                                                              >  2:478455/1‑138 (MQ=255)                                                                                gaTATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATAccc                                                       <  2:306065/139‑1 (MQ=255)                                                                                gaTATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATAccc                                                       <  1:242250/139‑1 (MQ=255)                                                                                 atatCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCgg                                                                               >  2:572436/1‑114 (MQ=255)                                                                                 atatCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCgg                                                                               <  1:572436/114‑1 (MQ=255)                                                                                 atatCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCa                                                      <  1:621033/139‑1 (MQ=255)                                                                                      cAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGc                                                 <  1:478455/139‑1 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGc                                                                                                                                <  1:74212/57‑1 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGc                                                                                                                                >  2:74212/1‑57 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGca                                                >  2:71866/1‑137 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGca                                                >  1:164162/1‑137 (MQ=255)                                                                                           cAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCa                                                                                           >  2:85363/1‑92 (MQ=255)                                                                                           cAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCa                                                                                           <  1:85363/92‑1 (MQ=255)                                                                                            agagGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGcc                                           <  1:71866/139‑1 (MQ=255)                                                                                                   ggCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGgag                                                                                                 >  2:156310/1‑78 (MQ=255)                                                                                                   ggCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGgag                                                                                                 <  1:156310/78‑1 (MQ=255)                                                                                                   ggCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAActcc                                     <  2:372623/138‑1 (MQ=255)                                                                                                          caAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGa                                                                                                                        >  2:199196/1‑48 (MQ=255)                                                                                                          caAGCACAATCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGa                                                                                                                        <  1:199196/48‑1 (MQ=255)                                                                                                                   tCACGTCAGGCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACCGCCCACTCCTCTCCCAACGCTcgccg                    >  2:343560/1‑139 (MQ=255)                                                                                                                           ggCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTgttgtt            <  2:164162/139‑1 (MQ=255)                                                                                                                           ggCTGCGAAGAGAGGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTgttgtt            <  1:699313/139‑1 (MQ=255)                                                                                                                                     agagGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCACTACTACg  <  2:257552/139‑1 (MQ=255)                                                                                                                                     agagGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCACTACTACg  <  1:501759/139‑1 (MQ=255)                                                                                                                                     agagGGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCACTACTACg  <  1:122186/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGTGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCACTACTACG  >  NZ_CP009273/1551692‑1551962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑