| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,575,074 | A→G | intergenic (‑27/+25) | ydeM ← / ← ydeN | anaerobic sulfatase maturase/sulfatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,575,074 | 0 | A | G | 97.9% | 172.4 / ‑2.8 | 47 | intergenic (‑27/+25) | ydeM/ydeN | anaerobic sulfatase maturase/sulfatase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/23); minor base . (1/0); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGAAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAACTCTCTTACCACGCCTTGCAT > NZ_CP009273/1574946‑1575206 | catccatTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATccgcc > 2:581480/1‑139 (MQ=255) atTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATg < 2:517122/139‑1 (MQ=255) ttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAgg > 1:279340/1‑139 (MQ=255) tAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTa > 2:362698/1‑139 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATttcgct < 2:101865/139‑1 (MQ=255) gACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATttcgctt > 1:376877/1‑139 (MQ=255) aCTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATccg > 2:5930/1‑111 (MQ=255) aCTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATccg < 1:5930/111‑1 (MQ=255) tttttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGG‑GAAATCCGCCGCATGAAGGTTTAGTTATTtcgcttcgct > 2:49114/1‑137 (MQ=255) tttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTtcgcttcgct < 1:581480/136‑1 (MQ=255) aaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa < 2:210974/139‑1 (MQ=255) aaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa < 1:38162/139‑1 (MQ=255) tAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTa > 2:310710/1‑121 (MQ=255) tAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTa < 1:310710/121‑1 (MQ=255) tAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGt > 1:709851/1‑139 (MQ=255) tGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa < 1:542243/123‑1 (MQ=255) tGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa > 2:542243/1‑123 (MQ=255) aCATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGAtt > 1:77139/1‑138 (MQ=255) aCATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTT‑GCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTa > 1:50637/1‑139 (MQ=255) ttGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTAcc < 2:376877/139‑1 (MQ=255) tGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAg > 2:667405/1‑139 (MQ=255) gAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAg < 2:402456/137‑1 (MQ=255) gAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAg < 2:709851/137‑1 (MQ=255) gAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCg > 1:733244/1‑139 (MQ=255) cTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGtggc > 2:325316/1‑139 (MQ=255) aCGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGctgctg < 1:275056/138‑1 (MQ=255) cGTGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTc < 1:325316/139‑1 (MQ=255) tGCATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGa > 1:129061/1‑139 (MQ=255) cATCGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGAt > 1:282000/1‑138 (MQ=255) cGCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAAc > 2:242835/1‑139 (MQ=255) gCTATCTCGCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAAc < 1:242835/138‑1 (MQ=255) gCTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAACTCTCTTAcc < 2:129061/139‑1 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa > 2:233108/1‑70 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa > 2:120104/1‑70 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa < 1:120104/70‑1 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGa < 1:233108/70‑1 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTtctc > 2:724080/1‑86 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTtctc < 1:724080/86‑1 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAActct > 1:646876/1‑132 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAActct < 2:646876/132‑1 (MQ=255) cTCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAACTCTCTTACCa > 2:710753/1‑139 (MQ=255) tCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGc < 1:321138/61‑1 (MQ=255) tCAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGc > 2:321138/1‑61 (MQ=255) cAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGCTATTGTTAAACTTCTCCTGATTTAcc > 2:67882/1‑94 (MQ=255) cAATAAGGCGGCGGGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTAcc < 1:67882/94‑1 (MQ=255) cggcggGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAACTCTCTTACCACGCCTTGCAt < 2:545867/139‑1 (MQ=255) cggcggGAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAACTCTCTTACCACGCCTTGCAt < 1:362698/139‑1 (MQ=255) | CATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGGCGGCGGAAAAATCCGCCGCATGAAGGTTTAGTTATTTCGCTTCGCTTAGTGCTTTCTTGATATTGTTAAACTTCTCCTGATTTACCTCGCTAAGCGGTGGCTGGCTGCTGTCGATAAACTCTCTTACCACGCCTTGCAT > NZ_CP009273/1574946‑1575206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |