Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,679,189 A→G N225S (AAC→AGC)  tus → DNA replication terminus site‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,679,1890AG100.0% 124.6 / NA 33N225S (AAC→AGC) tusDNA replication terminus site‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

GTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAA  >  NZ_CP009273/1679063‑1679315
                                                                                                                              |                                                                                                                              
gTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTaa                                                                                                                     >  1:343994/1‑138 (MQ=255)
gTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTaa                                                                                                                     >  1:719049/1‑138 (MQ=255)
                 caGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGa                                                                                                   <  1:505110/139‑1 (MQ=255)
                                        accacGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                            <  2:549085/139‑1 (MQ=255)
                                                 tgtCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACa                                                                     >  2:540700/1‑137 (MQ=255)
                                                   tCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAgg                                                                 <  1:424516/139‑1 (MQ=255)
                                                   tCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAg                                                                  >  2:11638/1‑138 (MQ=255)
                                                         cGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGcc                                                                                  >  2:435936/1‑116 (MQ=255)
                                                         cGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGcc                                                                                  <  1:435936/116‑1 (MQ=255)
                                                         cGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGGCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCa                                                           <  1:186950/139‑1 (MQ=255)
                                                           tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                            >  1:182624/1‑120 (MQ=255)
                                                           tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                            <  2:182624/120‑1 (MQ=255)
                                                           tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                          >  2:85306/1‑138 (MQ=255)
                                                           tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                          >  1:227984/1‑138 (MQ=255)
                                                             gACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaaaaa                                                       >  1:109883/1‑139 (MQ=255)
                                                                     aggagTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCGACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTcc                                                >  2:563911/1‑138 (MQ=255)
                                                                       gagTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTc                                                                                                         >  1:82072/1‑79 (MQ=255)
                                                                       gagTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTc                                                                                                         <  2:82072/79‑1 (MQ=255)
                                                                         gTGGAAAAGAAAACTGGAGCGAGAGTATCAGGATACCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGATATCAAAAACAAGTCCAacac                                           <  2:602871/139‑1 (MQ=255)
                                                                            gCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGcc                                        <  1:85306/139‑1 (MQ=255)
                                                                            gCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGcc                                        >  2:546982/1‑139 (MQ=255)
                                                                            gCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                         <  1:540700/138‑1 (MQ=255)
                                                                            gCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                         <  2:258260/138‑1 (MQ=255)
                                                                              aaaGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTg                                      <  1:546982/139‑1 (MQ=255)
                                                                                aGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGcc                                    <  2:343994/139‑1 (MQ=255)
                                                                                  aaaaCCGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCt                                  <  2:293036/139‑1 (MQ=255)
                                                                                                            tCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                            >  1:189312/1‑71 (MQ=255)
                                                                                                            tCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                            <  2:189312/71‑1 (MQ=255)
                                                                                                            tCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCg        >  2:552567/1‑139 (MQ=255)
                                                                                                             cGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCgg       >  1:267741/1‑139 (MQ=255)
                                                                                                                  ccctgccACAGAGCGCGAAGTTAAAAATCAAACGt                                                                                                          <  2:681392/35‑1 (MQ=255)
                                                                                                                  ccctgccACAGAGCGCGAAGTTAAAAATCAAACGt                                                                                                          >  1:681392/1‑35 (MQ=255)
                                                                                                                  ccctgccACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATaa  >  1:509375/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                              
GTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAA  >  NZ_CP009273/1679063‑1679315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑