| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,690,676 | A→C | intergenic (‑348/+43) | uidA ← / ← uidR | beta‑glucuronidase/DNA‑binding transcriptional regulator UidR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,690,676 | 0 | A | C | 94.4% | 124.7 / ‑1.6 | 36 | intergenic (‑348/+43) | uidA/uidR | beta‑glucuronidase/DNA‑binding transcriptional regulator UidR |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (18/16); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGC > NZ_CP009273/1690540‑1690808 | aaaTAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACcg < 2:544403/139‑1 (MQ=255) aaGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCggg > 2:190531/1‑139 (MQ=255) aGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCggg > 1:509280/1‑138 (MQ=255) aGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGc < 1:190531/139‑1 (MQ=255) gCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGca > 1:142782/1‑139 (MQ=255) cGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGt > 2:420409/1‑138 (MQ=255) gAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAagaga < 1:410257/139‑1 (MQ=255) aaCGATAAACTGTGGTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGTTGTGGTCGTAGAGCACAGTTATCGCCAGCCCGCCGAAAAaga > 2:658554/1‑136 (MQ=255) aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAagagag < 2:389831/139‑1 (MQ=255) aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGaa > 2:581698/1‑139 (MQ=255) aCTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAg > 2:461534/1‑139 (MQ=255) cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAg < 1:148058/109‑1 (MQ=255) cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAg > 2:148058/1‑109 (MQ=255) aCATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGt < 1:646661/138‑1 (MQ=255) tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGa > 1:204039/1‑138 (MQ=255) aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGc > 2:261876/1‑79 (MQ=255) aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGc < 1:261876/79‑1 (MQ=255) tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt > 1:621507/1‑119 (MQ=255) tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt < 2:621507/119‑1 (MQ=255) aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt > 2:474308/1‑117 (MQ=255) aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt < 1:474308/117‑1 (MQ=255) aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatca > 2:126420/1‑139 (MQ=255) aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatca > 2:126346/1‑139 (MQ=255) tttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAagaga > 1:724649/1‑100 (MQ=255) tttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAagaga < 2:724649/100‑1 (MQ=255) ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCt > 1:536844/1‑139 (MQ=255) aTTTTTGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc < 2:536844/139‑1 (MQ=255) tttGCACAGAGAAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAg < 1:442505/138‑1 (MQ=255) agaAATTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCt > 2:391853/1‑139 (MQ=255) aaTTACGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCATACGACGCTTCACGCGCCAGGTCTTCCGCTTCg < 1:665130/139‑1 (MQ=255) aCGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata < 1:692615/138‑1 (MQ=255) cGATATGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc < 2:444198/139‑1 (MQ=255) atatGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg < 1:72908/106‑1 (MQ=255) atatGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg > 2:72908/1‑106 (MQ=255) tatGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc < 1:126346/139‑1 (MQ=255) atGAACCGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc > 2:50710/1‑138 (MQ=255) | AAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGC > NZ_CP009273/1690540‑1690808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |