Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	59,627	C→A	intergenic (‑153/+60)	BW25113_RS25400 ← / ← rluA	transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	59,627	0	C	A	100.0%	158.6 / NA	39	intergenic (‑153/+60)	BW25113_RS25400/rluA	transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/23);  total (16/23)

TAAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGT  >  NZ_CP009273/59498‑59762                                                                                                                                  |                                                                                                                                        taAGAGACGGTCGACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGAt                                                                                                                                 <  2:664915/138‑1 (MQ=255)   agagACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGAttt                                                                                                                               <  2:587313/138‑1 (MQ=255)   agagACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGAttt                                                                                                                               <  1:452089/138‑1 (MQ=255)    gagaCGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTAt                                                                                                                             <  1:76924/139‑1 (MQ=255)           tCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                         >  2:549014/1‑136 (MQ=255)               cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                                  <  1:432148/139‑1 (MQ=255)                 ggTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCa                                                                                                                 <  2:672040/138‑1 (MQ=255)                 ggTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCa                                                                                                                 >  1:672040/1‑138 (MQ=255)                     aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                                >  1:224207/1‑135 (MQ=255)                     aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                                <  2:601545/135‑1 (MQ=255)                     aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                                <  2:224207/135‑1 (MQ=255)                     aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                                >  1:601545/1‑135 (MQ=255)                     aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                            >  2:433054/1‑139 (MQ=255)                           aaGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGAt                                                                                                      <  1:433054/139‑1 (MQ=255)                            aGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCggg                                                                                                                                    >  2:763310/1‑108 (MQ=255)                            aGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCggg                                                                                                                                    <  1:763310/108‑1 (MQ=255)                             gTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGa                                                                                                    <  1:426345/139‑1 (MQ=255)                                cAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAggg                                                                                                 >  1:411453/1‑139 (MQ=255)                                    aaTTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGtata                                                                                             <  2:580820/139‑1 (MQ=255)                                               gAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGa                                                                                  <  1:638997/139‑1 (MQ=255)                                                aGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGa                                                                                  >  1:330370/1‑138 (MQ=255)                                                aGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGa                                                                                  <  2:330370/138‑1 (MQ=255)                                                       aGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTa                                                                           >  2:243637/1‑138 (MQ=255)                                                            aTTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                                  >  1:405275/1‑94 (MQ=255)                                                            aTTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                                  <  2:405275/94‑1 (MQ=255)                                                                   aaTGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGgc                                                              >  2:32977/1‑139 (MQ=255)                                                                         aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTa                                                        <  2:554085/139‑1 (MQ=255)                                                                          aGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaa                                                       <  1:549014/139‑1 (MQ=255)                                                                          aGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTa                                                        >  2:82041/1‑138 (MQ=255)                                                                              tAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGAt                                                                                                      >  2:275979/1‑88 (MQ=255)                                                                              tAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGAt                                                                                                      <  1:275979/88‑1 (MQ=255)                                                                                                 aaaGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCggg                                                                                                                                    <  1:427069/39‑1 (MQ=255)                                                                                                 aaaGCAGTTTGGCAGAAACAAAAAATCCCCGGAACCggg                                                                                                                                    >  2:427069/1‑39 (MQ=255)                                                                                                               gAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGt                  <  2:726410/139‑1 (MQ=255)                                                                                                                aaaCAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGt                  >  2:649456/1‑138 (MQ=255)                                                                                                                  aCAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCaa               <  1:126368/139‑1 (MQ=255)                                                                                                                  aCAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCaa               >  1:528542/1‑139 (MQ=255)                                                                                                                    aaaaaaTCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACa             <  1:243637/139‑1 (MQ=255)                                                                                                                               ggAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGt  <  2:411453/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TAAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGT  >  NZ_CP009273/59498‑59762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑