Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,695,442	T→G	F77V (TTT→GTT)	malY →	bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,695,442	0	T	G	100.0%	204.2 / NA	54	F77V (TTT→GTT)	malY	bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/30);  total (24/30)

ACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACC  >  NZ_CP009273/1695306‑1695569                                                                                                                                         |                                                                                                                                aCCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGgtt                                                                                                                               >  2:313915/1‑139 (MQ=255)  ccGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGgttt                                                                                                                              <  1:533277/139‑1 (MQ=255)     ttCACGATTTCAGACATGGATTTTGCCCCTGCCCCCTGCATTATAGAGGCGCTGAATAAGCGCCTGATGCACGGCGTATGTGGCTACAGCAGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACGGGGTTTccg                                                                                                                           >  2:318911/1‑138 (MQ=255)     ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCa                                                                                                                           <  2:197720/139‑1 (MQ=255)      tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAc                                                                                                                          >  1:491590/1‑139 (MQ=255)      tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAc                                                                                                                          >  1:60566/1‑139 (MQ=255)        aCGATTTCAGACATGGATTTTGCCACTGCCCCATGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                         <  1:359472/138‑1 (MQ=255)                      ggATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGc                                                                                                           >  2:181255/1‑138 (MQ=255)                                 cTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATtct                                                                                                 >  1:439519/1‑137 (MQ=255)                                    cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             <  1:627285/138‑1 (MQ=255)                                    cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             <  2:581838/138‑1 (MQ=255)                                    cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             <  1:89638/138‑1 (MQ=255)                                    cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             <  1:521606/138‑1 (MQ=255)                                      cccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACgg                                                                                          <  1:755906/139‑1 (MQ=255)                                       ccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACggt                                                                                         <  1:51060/139‑1 (MQ=255)                                         tGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATtctc                                                                                                <  1:215847/130‑1 (MQ=255)                                         tGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATtctc                                                                                                >  2:215847/1‑130 (MQ=255)                                             ttATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                                                                                   <  2:362091/139‑1 (MQ=255)                                              tATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTAt                                                                                  >  2:451561/1‑139 (MQ=255)                                              tATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTAt                                                                                  >  1:481685/1‑139 (MQ=255)                                                tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACgg                                                                                          >  1:167163/1‑129 (MQ=255)                                                tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACgg                                                                                          <  2:167163/129‑1 (MQ=255)                                                   aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGccc                                                                             >  1:124077/1‑139 (MQ=255)                                                   aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGccc                                                                             >  1:409933/1‑139 (MQ=255)                                                     gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                         <  1:44913/93‑1 (MQ=255)                                                     gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                         >  2:44913/1‑93 (MQ=255)                                                       gcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCt                                                                         <  2:590208/139‑1 (MQ=255)                                                        cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                                                                                   <  1:479127/128‑1 (MQ=255)                                                        cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                                                                                   >  2:479127/1‑128 (MQ=255)                                                          gAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                         <  2:76211/88‑1 (MQ=255)                                                          gAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                         >  1:76211/1‑88 (MQ=255)                                                           aaTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTc                                                                      <  2:351648/138‑1 (MQ=255)                                                                  gcCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTatat                                                              <  2:473018/139‑1 (MQ=255)                                                                     tGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             <  1:7434/105‑1 (MQ=255)                                                                     tGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             >  2:7434/1‑105 (MQ=255)                                                                     tGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             >  1:426971/1‑105 (MQ=255)                                                                     tGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                             <  2:426971/105‑1 (MQ=255)                                                                       aTGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGttt                                                         <  2:463975/139‑1 (MQ=255)                                                                               cGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACt                                                  >  1:134691/1‑138 (MQ=255)                                                                                        cTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                                        <  2:595639/139‑1 (MQ=255)                                                                                        cTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                                        <  1:451561/139‑1 (MQ=255)                                                                                           cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCa                                                                     <  1:527805/107‑1 (MQ=255)                                                                                           cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCa                                                                     >  2:527805/1‑107 (MQ=255)                                                                                            aGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGt                                    >  2:313291/1‑139 (MQ=255)                                                                                                              gAGTTTCTCGCGTCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTg                        <  2:468683/133‑1 (MQ=255)                                                                                                              gAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTg                        >  1:468683/1‑133 (MQ=255)                                                                                                                             aTTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcacaca    <  1:313291/138‑1 (MQ=255)                                                                                                                               tGCCCACGGGGTTTCCCCCCAGCATTCCCCCGCCATCTATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACAcccc  <  2:491590/138‑1 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGcc                                                                              <  2:158834/55‑1 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGcc                                                                              >  1:158834/1‑55 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                                        <  1:480010/93‑1 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                                        >  2:480010/1‑93 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCg                  >  1:315649/1‑115 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCg                  <  2:315649/115‑1 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcacaca    >  1:390894/1‑129 (MQ=255)                                                                                                                                      tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcacaca    <  2:390894/129‑1 (MQ=255)                                                                                                                                         |                                                                                                                                ACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACC  >  NZ_CP009273/1695306‑1695569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑