Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,703,908 T→G G170G (GGT→GGG)  rsxD → electron transport complex subunit RsxD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,703,9080TG97.2% 135.7 / ‑3.7 36G170G (GGT→GGG) rsxDelectron transport complex subunit RsxD
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (17/18);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGTGGTCTTACTGATCTCCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGTGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTG  >  NZ_CP009273/1703782‑1704043
                                                                                                                              |                                                                                                                                       
tgtgGTCTTACTGATCTCCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGaa                                                                                                                              <  2:765180/138‑1 (MQ=255)
tgtgGTCTTACTGATCTCCTTCCCCGTGCAGATGACCAGCTGG‑TACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAaca                                                                                                                            <  1:305329/139‑1 (MQ=255)
    gTCTTACTGATCTCCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAacaca                                                                                                                          <  2:118178/138‑1 (MQ=255)
       ttACTGATCTCCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAacaca                                                                                                                          <  1:143201/135‑1 (MQ=255)
       ttACTGATCTCCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAacaca                                                                                                                          >  2:143201/1‑135 (MQ=255)
               ctcCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAgg                                                                                                               >  2:485002/1‑138 (MQ=255)
               ctcCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAgg                                                                                                               >  1:39035/1‑138 (MQ=255)
                    tCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGa                                                                                                         >  1:564516/1‑139 (MQ=255)
                     ccccGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGAt                                                                                                        >  1:397548/1‑139 (MQ=255)
                       ccGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGaa                                                                                                                              <  1:15629/115‑1 (MQ=255)
                       ccGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGaa                                                                                                                              >  2:15629/1‑115 (MQ=255)
                       ccGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATgg                                                                                                      >  1:431180/1‑139 (MQ=255)
                       ccGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATgg                                                                                                      >  2:275878/1‑139 (MQ=255)
                          tGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCAt                                                                                                   >  1:670684/1‑139 (MQ=255)
                          tGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCAt                                                                                                   >  1:30030/1‑139 (MQ=255)
                                     aGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGa                                                                                        <  1:355242/139‑1 (MQ=255)
                                       cTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGaca                                                                                      >  2:504974/1‑139 (MQ=255)
                                         ggTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTa                                                                                                                       >  1:257889/1‑104 (MQ=255)
                                         ggTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTa                                                                                                                       <  2:257889/104‑1 (MQ=255)
                                         ggTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACAcc                                                                                    >  1:524656/1‑139 (MQ=255)
                                          gTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCg                                                                                   <  2:448461/139‑1 (MQ=255)
                                                gCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGAt                                                                             <  1:275878/139‑1 (MQ=255)
                                                 ccACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATa                                                                            >  1:361940/1‑139 (MQ=255)
                                                 ccACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATa                                                                            <  2:296755/139‑1 (MQ=255)
                                                        aaaTTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGTGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTaa                                                                     <  2:652383/139‑1 (MQ=255)
                                                                         ccTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGcc                                                     <  2:90620/138‑1 (MQ=255)
                                                                                   tCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCgg                                           <  2:455794/138‑1 (MQ=255)
                                                                                       cGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGaa                                      <  1:180061/139‑1 (MQ=255)
                                                                                                 gTTATTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGc                            <  2:560301/139‑1 (MQ=255)
                                                                                                    aTTTTTAGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCaa                          >  1:59702/1‑138 (MQ=255)
                                                                                                          aGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCa                                                                                                    <  2:490469/58‑1 (MQ=255)
                                                                                                          aGCGGTCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCa                                                                                                    >  1:490469/1‑58 (MQ=255)
                                                                                                               tCATACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTa               >  2:479230/1‑138 (MQ=255)
                                                                                                                 aTACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAg            <  2:361940/139‑1 (MQ=255)
                                                                                                                 aTACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAg            <  2:203297/139‑1 (MQ=255)
                                                                                                                 aTACCGCCAGTGGGGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAg            <  1:59440/139‑1 (MQ=255)
                                                                                                                           tgggggtgATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTg  >  2:345740/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                       
TGTGGTCTTACTGATCTCCTTCCCCGTGCAGATGACCAGCTGGTTACCGCCACATGAAATTGCGGTCAACATCCCTGGTTTTATCGACGCCATCCAGGTTATTTTTAGCGGTCATACCGCCAGTGGTGGTGATATGAACACACTACGCTTAGGTATTGATGGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTG  >  NZ_CP009273/1703782‑1704043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑