Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,747,881	A→C	G97G (GGT→GGG)	ydhV ←	aldehyde ferredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,747,881	0	A	C	100.0%	117.8 / NA	32	G97G (GGT→GGG)	ydhV	aldehyde ferredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/12);  total (20/12)

ATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTG  >  NZ_CP009273/1747756‑1748016                                                                                                                              |                                                                                                                                        atGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGtt                                                                                                                             >  2:98303/1‑138 (MQ=255)          tATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATc                                                                                                                                  >  2:51427/1‑124 (MQ=255)          tATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATc                                                                                                                                  <  1:51427/124‑1 (MQ=255)          tATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCgg                                                                                                                   <  1:654442/139‑1 (MQ=255)            tCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCgg                                                                                                                   >  2:654442/1‑137 (MQ=255)                   aaTTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGa                                                                                                          <  1:355028/139‑1 (MQ=255)                   aaTTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGa                                                                                                          <  1:549488/139‑1 (MQ=255)                                   cAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCtt                                                                                           >  2:575353/1‑138 (MQ=255)                                                         aTCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc                                                                    >  1:303948/1‑139 (MQ=255)                                                         aTCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc                                                                    >  2:440961/1‑139 (MQ=255)                                                          tCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCg                                                                   >  2:619737/1‑139 (MQ=255)                                                           cgacgaCTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                                                              >  1:187519/1‑95 (MQ=255)                                                           cgacgaCTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                                                              <  2:187519/95‑1 (MQ=255)                                                           cgacgaCTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCgg                                                                  >  2:727187/1‑139 (MQ=255)                                                             acgacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTa                                                                >  1:77727/1‑139 (MQ=255)                                                               gacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACt                                                              <  1:619737/139‑1 (MQ=255)                                                               gacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACt                                                              <  2:682785/139‑1 (MQ=255)                                                                 cTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTc                                                            >  1:143236/1‑139 (MQ=255)                                                                 cTAAATTTCCTTTGGTAAAAGTAGAAAGCGAGGTGATATTTACGAGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTc                                                            >  1:198997/1‑139 (MQ=255)                                                                    aaTTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCg                                                           <  2:66238/137‑1 (MQ=255)                                                                             tGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATaa                                                 <  1:440961/138‑1 (MQ=255)                                                                              ggTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAAtt                                               >  1:356658/1‑139 (MQ=255)                                                                                                ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATg                             >  1:736577/1‑139 (MQ=255)                                                                                                ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATg                             >  2:703300/1‑139 (MQ=255)                                                                                                ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATg                             >  1:75366/1‑139 (MQ=255)                                                                                                           cgcgAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACaa                   <  2:303948/138‑1 (MQ=255)                                                                                                                 aaCTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                                                              >  2:594133/1‑41 (MQ=255)                                                                                                                 aaCTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                                                              <  1:594133/41‑1 (MQ=255)                                                                                                                 aaCTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACttt             >  1:495980/1‑138 (MQ=255)                                                                                                                           ggCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACt                                                              >  2:503860/1‑79 (MQ=255)                                                                                                                           ggCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACt                                                              <  1:503860/79‑1 (MQ=255)                                                                                                                           ggCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTg  >  2:286484/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                        ATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTG  >  NZ_CP009273/1747756‑1748016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑