Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,806,054	T→C	S288S (AGT→AGC)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,806,054	0	T	C	100.0%	180.1 / NA	50	S288S (AGT→AGC)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/24);  total (26/24)

AATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTT  >  NZ_CP009273/1805922‑1806188                                                                                                                                     |                                                                                                                                       aaTGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTg                                                                                                                                  >  2:72987/1‑139 (MQ=255)      ccAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             >  1:301130/1‑139 (MQ=255)          aGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTc                                                                                                                         >  2:595067/1‑139 (MQ=255)              gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  2:578856/138‑1 (MQ=255)              gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  1:734953/138‑1 (MQ=255)              gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  1:72987/138‑1 (MQ=255)              gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  1:595067/138‑1 (MQ=255)               ttGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      >  2:734953/1‑137 (MQ=255)                        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  2:89918/138‑1 (MQ=255)                        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  2:497905/138‑1 (MQ=255)                        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  2:535713/138‑1 (MQ=255)                        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  2:222384/138‑1 (MQ=255)                        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  1:20653/138‑1 (MQ=255)                        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  2:687009/138‑1 (MQ=255)                               gCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCt                                                                                                     >  2:625597/1‑138 (MQ=255)                                cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  1:406508/120‑1 (MQ=255)                                cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      >  2:406508/1‑120 (MQ=255)                                cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      >  1:326045/1‑120 (MQ=255)                                cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  2:326045/120‑1 (MQ=255)                                           caAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCtt                                                                                         >  2:548577/1‑138 (MQ=255)                                                tGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             >  2:505775/1‑97 (MQ=255)                                                tGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             <  1:505775/97‑1 (MQ=255)                                                  ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGcc                                                                                 >  2:581907/1‑139 (MQ=255)                                                  ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                                  <  1:482938/138‑1 (MQ=255)                                                           gTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtg                                                                        <  2:6317/139‑1 (MQ=255)                                                              gTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                      <  1:548577/138‑1 (MQ=255)                                                                              gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            >  2:323296/1‑84 (MQ=255)                                                                              gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                            <  1:323296/84‑1 (MQ=255)                                                                              gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGaa                                                     >  2:133747/1‑139 (MQ=255)                                                                               tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAt                                                    >  1:392534/1‑139 (MQ=255)                                                                               tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAt                                                    >  1:74207/1‑139 (MQ=255)                                                                                   ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTgg                                                <  1:8607/139‑1 (MQ=255)                                                                                           gTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGcaca                                         >  2:301217/1‑138 (MQ=255)                                                                                               gCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcg                                     <  2:762488/138‑1 (MQ=255)                                                                                                     cATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCgtcgtc                              >  1:176022/1‑139 (MQ=255)                                                                                                        ggCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGt                                                                                                                 <  2:294328/53‑1 (MQ=255)                                                                                                        ggCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGt                                                                                                                 >  1:294328/1‑53 (MQ=255)                                                                                                         gCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTggg                          <  2:74207/139‑1 (MQ=255)                                                                                                                    ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCa                                                          <  2:374023/96‑1 (MQ=255)                                                                                                                    ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCa                                                          >  1:374023/1‑96 (MQ=255)                                                                                                                    ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGCAGCCCAAACGCGTCGTCTGGGCGTTCCTGAAt               >  2:369659/1‑139 (MQ=255)                                                                                                                    ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAAt               >  1:620570/1‑139 (MQ=255)                                                                                                                      cATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                      <  1:729155/82‑1 (MQ=255)                                                                                                                      cATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                      >  2:729155/1‑82 (MQ=255)                                                                                                                      cATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATcc             <  2:392534/139‑1 (MQ=255)                                                                                                                      cATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATc              >  2:518931/1‑138 (MQ=255)                                                                                                                         tAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATc          >  2:195590/1‑139 (MQ=255)                                                                                                                         tAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCATCTGGGCGTTCCTGAATCCATc          >  1:110656/1‑139 (MQ=255)                                                                                                                               cGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGt    >  1:438505/1‑139 (MQ=255)                                                                                                                                 tGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGttt  >  2:545317/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       AATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTT  >  NZ_CP009273/1805922‑1806188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑