| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,848,241 | T→G | intergenic (‑5/+112) | ydjE ← / ← ydjF | MFS transporter/DeoR/GlpR family DNA‑binding transcription regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,848,241 | 0 | T | G | 92.6% | 91.3 / ‑0.2 | 27 | intergenic (‑5/+112) | ydjE/ydjF | MFS transporter/DeoR/GlpR family DNA‑binding transcription regulator |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (12/13); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01 | |||||||||||
CGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGC > NZ_CP009273/1848107‑1848365 | cgacATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCt < 2:664912/139‑1 (MQ=255) aTTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGa > 1:407287/1‑139 (MQ=255) aCTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAg > 1:506516/1‑139 (MQ=255) aCTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACtt > 2:35935/1‑139 (MQ=255) tAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGc > 1:731429/1‑139 (MQ=255) ataataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCtt > 1:684395/1‑139 (MQ=255) ccAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatca < 1:367508/138‑1 (MQ=255) cAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGAtt < 2:596526/88‑1 (MQ=255) cAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGAtt > 1:596526/1‑88 (MQ=255) aTAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTACATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt > 1:422281/1‑138 (MQ=255) tAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCa < 1:28257/139‑1 (MQ=255) aTGAACACGGGCCACAGGCATGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTACTAAAAAGGTAGCAATTGCGGCAAATTCACCTGCCGCATCTTTATCATCATTTTATTTCaa < 2:628954/138‑1 (MQ=255) tGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAgg > 2:699240/1‑139 (MQ=255) aaaCCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgcg < 1:44711/139‑1 (MQ=255) gggCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGa < 1:699240/139‑1 (MQ=255) gggCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGa < 1:55921/139‑1 (MQ=255) aGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaat > 2:767072/1‑138 (MQ=255) aGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACgt < 1:35935/139‑1 (MQ=255) cgcCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGcc < 1:757150/77‑1 (MQ=255) cgcCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGcc > 2:757150/1‑77 (MQ=255) cgcCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTat > 2:150427/1‑87 (MQ=255) cgcCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTat < 1:150427/87‑1 (MQ=255) cgcCAATTTGATCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCtt > 1:317423/1‑138 (MQ=255) tCATATTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCacac < 2:684395/139‑1 (MQ=255) atTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCg < 2:390657/63‑1 (MQ=255) atTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCg > 1:390657/1‑63 (MQ=255) atTGTTCCATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGc > 1:723915/1‑139 (MQ=255) | CGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGC > NZ_CP009273/1848107‑1848365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |