| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 63,109 | G→T | R52R (CGC→CGA) | rapA ← | RNA polymerase‑associated protein RapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 63,109 | 0 | G | T | 97.8% | 165.0 / ‑3.6 | 45 | R52R (CGC→CGA) | rapA | RNA polymerase‑associated protein RapA |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (28/16); total (29/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
GCCGGACTCTTCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACGCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCTGATCCAGCGT > NZ_CP009273/62983‑63244 | gCCGGACTCTTCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACgggg < 1:63287/139‑1 (MQ=255) gACTCTTCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAAt > 1:546884/1‑139 (MQ=255) gACTCTTCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAAt > 2:44567/1‑139 (MQ=255) ttCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACtg > 2:755004/1‑139 (MQ=255) ttCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACtg > 2:163883/1‑139 (MQ=255) ttCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACtg > 2:340600/1‑139 (MQ=255) ttCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACtg > 1:390725/1‑139 (MQ=255) ccAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTa > 1:702505/1‑139 (MQ=255) aGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTa > 2:518547/1‑137 (MQ=255) aGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTa > 2:698628/1‑137 (MQ=255) gCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGa < 2:161144/139‑1 (MQ=255) tACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACGCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGtt > 1:725642/1‑139 (MQ=255) atatAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCAc > 1:352199/1‑139 (MQ=255) gCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGtttt < 2:222928/126‑1 (MQ=255) gCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGtttt > 1:222928/1‑126 (MQ=255) cAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATgg > 1:251067/1‑138 (MQ=255) cAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATgg > 1:543079/1‑138 (MQ=255) gCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGaaa < 1:518547/139‑1 (MQ=255) aCTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGtt < 1:44567/138‑1 (MQ=255) cttcttCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTcg < 2:352199/139‑1 (MQ=255) ttcGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCa > 1:457469/1‑139 (MQ=255) tcGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACgg > 1:750634/1‑70 (MQ=255) tcGACTTGCATCTGCCAGCCGACATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACgg < 2:750634/70‑1 (MQ=255) cATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGa < 1:161529/85‑1 (MQ=255) cATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGa > 2:161529/1‑85 (MQ=255) ccGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTc > 2:532672/1‑139 (MQ=255) ccGTCATGGCTGGTAATGGCATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTc > 1:328594/1‑139 (MQ=255) gTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCa < 1:134550/139‑1 (MQ=255) gTAATGGTATCGCCAGGGGTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGTCGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTGCGCGCATCCACCGCGACAACGGTTCCAGGTCCCAAt < 2:373601/138‑1 (MQ=255) tATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGaaa < 2:642912/80‑1 (MQ=255) tATCACCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGaaa > 1:642912/1‑80 (MQ=255) aCCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGt < 1:755004/138‑1 (MQ=255) aCCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTa > 2:421572/1‑139 (MQ=255) aCCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTa > 1:459142/1‑139 (MQ=255) aCCAGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTa > 1:99118/1‑139 (MQ=255) aGGGTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCg < 2:459142/139‑1 (MQ=255) gggTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGc > 1:542412/1‑139 (MQ=255) gggTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGc < 2:328594/139‑1 (MQ=255) gggTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGc < 2:702505/139‑1 (MQ=255) ggTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCt > 2:120594/1‑139 (MQ=255) ggTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGCGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCt > 2:377966/1‑139 (MQ=255) ggTTGAACATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGAAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCt > 1:38177/1‑139 (MQ=255) cATCACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCTGATCCAg < 2:542412/139‑1 (MQ=255) cACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCTGATCCAGCGt > 2:219261/1‑139 (MQ=255) cACTCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCTGATCCAGCGt > 2:21732/1‑139 (MQ=255) | GCCGGACTCTTCAGTATCCAGGCGAGTACCGATATAGGTCAGCAAGCCATTTTCTTCTTTTACTTCTTCGACTTGCATCTGCCAGCCGTCATGGCTGGTAATGGTATCACCAGGGTTGAACATCACGCGGGTCACGGGGGAATCACTGCGTGCGTACAGACGGTTTTCACCAGTAGATGGGAAAAGTAAAGTGACAGTTCGCGCATCCACCGCGACAACGGTTCCAAGTCCCAATTCGCTTTCTGTATCGCTGATCCAGCGT > NZ_CP009273/62983‑63244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |