Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,865,194	A→C	N185H (AAC→CAC)	cdgI →	GGDEF domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,865,194	0	A	C	100.0%	137.1 / NA	35	N185H (AAC→CAC)	cdgI	GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/14);  total (21/14)

TAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTC  >  NZ_CP009273/1865060‑1865329                                                                                                                                       |                                                                                                                                        tAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGaccaca                                                                                                                                      <  2:407113/138‑1 (MQ=255)      aTCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCtt                                                                                                                                 >  2:201335/1‑138 (MQ=255)              aCCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAAt                                                                                                                        <  1:431179/139‑1 (MQ=255)                  aTTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAACAAg                                                                                                                     >  1:400021/1‑138 (MQ=255)                          cAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACg                                                                                                            >  1:2527/1‑139 (MQ=255)                          cAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACg                                                                                                            >  2:190811/1‑139 (MQ=255)                               cacTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAAt                                                                                                       >  2:736271/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  1:635986/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  1:675725/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  1:35227/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  2:752966/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAAGGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  1:688296/1‑139 (MQ=255)                                       cAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGc                                                                                               >  2:578912/1‑139 (MQ=255)                                       cAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGc                                                                                               <  1:736271/139‑1 (MQ=255)                                              aGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCt                                                                                        >  1:7123/1‑139 (MQ=255)                                                          tttctattttctatATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttttt                                                                             >  2:300122/1‑138 (MQ=255)                                                                ttttctatATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGt                                                                         <  1:283529/136‑1 (MQ=255)                                                                            tctgCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTgg                                                           >  2:210458/4‑138 (MQ=255)                                                                            tCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcccc                                                                 <  1:710728/132‑1 (MQ=255)                                                                            tCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcccc                                                                 >  2:710728/1‑132 (MQ=255)                                                                                   aCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAAt                                                   >  1:576019/1‑139 (MQ=255)                                                                                       aGTTTTATTTGTTTAACCTCGCTTGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGa                                               <  2:495418/139‑1 (MQ=255)                                                                                       aGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGa                                               <  2:558441/139‑1 (MQ=255)                                                                                         ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                             <  1:201335/139‑1 (MQ=255)                                                                                               ttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                              <  1:752884/132‑1 (MQ=255)                                                                                               ttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                              >  2:752884/1‑132 (MQ=255)                                                                                                   ttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCt                                                                                              >  2:343312/1‑80 (MQ=255)                                                                                                   ttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCt                                                                                              <  1:343312/80‑1 (MQ=255)                                                                                                      aCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCt                                 <  2:626647/138‑1 (MQ=255)                                                                                                      aCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTg                                <  1:31477/139‑1 (MQ=255)                                                                                                           gCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACt                             <  2:35227/137‑1 (MQ=255)                                                                                                            cTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAtt                          >  1:161325/1‑139 (MQ=255)                                                                                                                       ttGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg                 <  2:635986/137‑1 (MQ=255)                                                                                                                       ttGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg                 <  1:300122/137‑1 (MQ=255)                                                                                                                               gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa       >  2:398372/1‑139 (MQ=255)                                                                                                                                    gACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTc  >  1:243126/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        TAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTC  >  NZ_CP009273/1865060‑1865329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑