| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,882,935 | A→C | Y357D (TAT→GAT) | fadD ← | long‑chain‑fatty‑acid‑‑CoA ligase FadD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,882,935 | 0 | A | C | 95.0% | 140.6 / ‑0.4 | 40 | Y357D (TAT→GAT) | fadD | long‑chain‑fatty‑acid‑‑CoA ligase FadD |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (22/16); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
GTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCA > NZ_CP009273/1882803‑1883066 | gtggtACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCtt < 2:355700/138‑1 (MQ=255) cATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGAt < 1:648584/138‑1 (MQ=255) atcatcatcatcCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCGGCAGATACTGt > 1:768738/1‑139 (MQ=255) atcatcCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCGGCAGATACTGTCCGGt < 2:768738/138‑1 (MQ=255) atcatcCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGt < 1:361299/138‑1 (MQ=255) tttGGCTTCCGACGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCtt < 1:147077/107‑1 (MQ=255) tttGGCTTCCGACGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCtt > 2:147077/1‑107 (MQ=255) ttGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGt < 2:299250/125‑1 (MQ=255) ttGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGt > 1:299250/1‑125 (MQ=255) tGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACg > 1:700058/1‑139 (MQ=255) tGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACg > 1:105266/1‑139 (MQ=255) cTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGctc < 1:125257/139‑1 (MQ=255) aTGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCcag < 2:225255/80‑1 (MQ=255) aTGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCcag > 1:225255/1‑80 (MQ=255) ccACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGc > 1:7257/1‑139 (MQ=255) aTGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCg > 1:597734/1‑139 (MQ=255) tGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGCCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCg > 1:92805/1‑138 (MQ=255) cAATATCATATGGTTTAACGCTGCCCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCCCCGCCTGCGGAAAGATg < 2:709501/139‑1 (MQ=255) aaTATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATg < 1:533006/138‑1 (MQ=255) tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATg > 1:657454/1‑136 (MQ=255) tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATg > 2:749355/1‑136 (MQ=255) tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATg > 2:270812/1‑136 (MQ=255) atGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTg > 2:361407/1‑137 (MQ=255) tGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGga < 2:7257/138‑1 (MQ=255) gTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGt < 1:581095/63‑1 (MQ=255) gTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGt > 2:581095/1‑63 (MQ=255) gTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGaaa > 1:96193/1‑139 (MQ=255) tAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATc > 2:481699/1‑139 (MQ=255) aaCGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATc > 2:242461/1‑138 (MQ=255) aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCtt < 2:35921/40‑1 (MQ=255) aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATCGCCtt > 1:35921/1‑40 (MQ=255) cGCTGACCAGCGGCGCACACTCGGTAAGCCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAg > 2:288200/1‑139 (MQ=255) aCCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAtcttct > 2:540069/1‑139 (MQ=255) aCCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAgctgct < 1:288200/139‑1 (MQ=255) aCCAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAgctgct > 1:419238/1‑139 (MQ=255) ccAGCGGCGCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGctgctg < 1:481699/139‑1 (MQ=255) gcggcgCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGaa < 2:419238/139‑1 (MQ=255) cggcgCACACTCGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAAc > 2:659665/1‑139 (MQ=255) cGGTAAGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGtt > 1:180681/1‑139 (MQ=255) aGGCCATCGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCa < 1:540069/139‑1 (MQ=255) | GTGGTACTTCATTATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCA > NZ_CP009273/1882803‑1883066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |