Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,913,668	C→A	G634G (GGC→GGA)	yebT →	lipid‑binding membrane homeostasis protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,913,668	0	C	A	100.0%	163.6 / NA	41	G634G (GGC→GGA)	yebT	lipid‑binding membrane homeostasis protein YebT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/20);  total (21/20)

GCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGG  >  NZ_CP009273/1913540‑1913801                                                                                                                                 |                                                                                                                                      gCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTa                                                                                                                             <  2:611126/139‑1 (MQ=255)  cATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTAc                                                                                                                            >  1:650135/1‑139 (MQ=255)  cATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTAc                                                                                                                            >  1:396083/1‑139 (MQ=255)   aTCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACg                                                                                                                           >  1:366173/1‑139 (MQ=255)    tCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGc                                                                                                                          >  2:594783/1‑139 (MQ=255)      cccGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCtt                                                                                                                        >  1:487918/1‑139 (MQ=255)             tcCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCtt                                                                                                                 >  2:351526/1‑139 (MQ=255)                               gCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTgg                                                                                               >  2:368980/1‑139 (MQ=255)                                   ttAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGt                                                                                           <  1:404295/139‑1 (MQ=255)                                              aaCCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCaa                                                                                <  2:650135/139‑1 (MQ=255)                                                ccTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAAtt                                                                              >  1:654077/1‑139 (MQ=255)                                                 cTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTc                                                                             >  1:120235/1‑139 (MQ=255)                                                       ggTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTAtc                                                                       <  1:221119/139‑1 (MQ=255)                                                         tGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTAtctc                                                                     <  2:654077/139‑1 (MQ=255)                                                         tGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTAtctc                                                                     <  2:620928/139‑1 (MQ=255)                                                           ccAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCgg                                                                   >  1:369734/1‑139 (MQ=255)                                                           ccAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCgg                                                                   >  2:36470/1‑139 (MQ=255)                                                              gcgcCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTAt                                                                <  2:396083/139‑1 (MQ=255)                                                                         cGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGGTTTCggg                                                      >  2:712911/1‑138 (MQ=255)                                                                         cGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGc                                                     <  2:366173/139‑1 (MQ=255)                                                                              aGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCa                                                    <  2:173566/135‑1 (MQ=255)                                                                              aGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCa                                                    >  1:173566/1‑135 (MQ=255)                                                                                   aTAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGAc                                           >  1:414624/1‑139 (MQ=255)                                                                                   aTAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGAc                                           >  2:696725/1‑139 (MQ=255)                                                                                                gTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGAt                               >  1:355075/1‑138 (MQ=255)                                                                                                 tATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGa                                <  1:594783/136‑1 (MQ=255)                                                                                                 tATGCTTCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTa                             >  2:738684/1‑139 (MQ=255)                                                                                                      ttCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCggg                                                      >  2:264301/1‑109 (MQ=255)                                                                                                      ttCCGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCggg                                                      <  1:264301/109‑1 (MQ=255)                                                                                                        ccGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACcgca                        <  2:297876/137‑1 (MQ=255)                                                                                                        ccGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACcgca                        <  2:120235/137‑1 (MQ=255)                                                                                                        ccGAAACAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACcgca                        <  1:696725/137‑1 (MQ=255)                                                                                                             aCAGCGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATg                 <  2:713771/139‑1 (MQ=255)                                                                                                                 cGGCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAg              >  1:317559/1‑138 (MQ=255)                                                                                                                  ggCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTAtct                                                                      <  2:156932/81‑1 (MQ=255)                                                                                                                  ggCCCGTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTAtct                                                                      >  1:156932/1‑81 (MQ=255)                                                                                                                       gTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGc       <  2:414624/139‑1 (MQ=255)                                                                                                                       gTGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGc       <  2:487918/139‑1 (MQ=255)                                                                                                                        tGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCCATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACcgca                        >  1:643052/1‑121 (MQ=255)                                                                                                                        tGCGGTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACcgca                        <  2:643052/121‑1 (MQ=255)                                                                                                                            gTTGGAGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAgg  <  2:468618/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      GCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGG  >  NZ_CP009273/1913540‑1913801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑