Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,919,822	T→C	F42F (TTT→TTC)	yobB →	carbon‑nitrogen hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,919,822	0	T	C	100.0%	205.1 / NA	53	F42F (TTT→TTC)	yobB	carbon‑nitrogen hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/31);  total (22/31)

GGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGT  >  NZ_CP009273/1919687‑1919953                                                                                                                                         |                                                                                                                                    ggAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCa                                                                                                                                  <  2:288482/139‑1 (MQ=255)     gTCTTTATGCGGTTCTGGAAAGTTGCACCAGCGCAATAGGAGCCTCGTAAAACCTCA‑TTACCGAAACAGGAAGCCCATCATCTGGAGTTTGTAAGGGCCGCCGCCAGGCACCAATGCCAGCTGTTGGTTTTCCCATCGc                                                                                                                              >  2:479028/1‑139 (MQ=255)              tCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTAc                                                                                                                     <  1:107027/139‑1 (MQ=255)                gTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACt                                                                                                                    <  2:504954/138‑1 (MQ=255)                gTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTg                                                                                                                   <  2:655137/139‑1 (MQ=255)                 ttCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGct                                                                                                                             >  2:273350/1‑128 (MQ=255)                 ttCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGct                                                                                                                             <  1:273350/128‑1 (MQ=255)                      gAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGgtgt                                                                                                             <  1:635207/139‑1 (MQ=255)                      gAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGgt                                                                                                               <  2:404551/137‑1 (MQ=255)                                  agcGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGa                                                                                                 <  2:562433/139‑1 (MQ=255)                                     gcAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGt                                                                                              >  1:285566/1‑139 (MQ=255)                                     gcAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGt                                                                                              >  1:26806/1‑139 (MQ=255)                                      cAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg                                                                                             <  1:709876/139‑1 (MQ=255)                                       aaTATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGc                                                                                            <  1:566901/139‑1 (MQ=255)                                             aGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                                                                                      <  1:452625/139‑1 (MQ=255)                                             aGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTc                                                                                       <  2:285566/138‑1 (MQ=255)                                              gCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCt                                                                                     >  1:231680/1‑139 (MQ=255)                                              gCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGCTTGAGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCt                                                                                     >  1:108488/1‑139 (MQ=255)                                                 tCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGc                                                                                            >  2:719304/1‑129 (MQ=255)                                                 tCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGc                                                                                            <  1:719304/129‑1 (MQ=255)                                                   gTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTg                                                                                                                   <  2:718527/104‑1 (MQ=255)                                                   gTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTg                                                                                                                   >  1:718527/1‑104 (MQ=255)                                                   gTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGtgtg                                                                                                            >  2:390988/1‑111 (MQ=255)                                                   gTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGtgtg                                                                                                            <  1:390988/111‑1 (MQ=255)                                                   gTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTTCCCTTCCTGccc                                                                                 <  1:521813/138‑1 (MQ=255)                                                      aaaCCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCAcc                                                                             <  1:613759/139‑1 (MQ=255)                                                          ctcactcaCCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAt                                                                         <  2:179404/139‑1 (MQ=255)                                                              ctcacCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctct                                                                     <  1:570420/139‑1 (MQ=255)                                                                 acCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctctc                                                                    <  2:231680/137‑1 (MQ=255)                                                                 acCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctctc                                                                    <  1:319950/137‑1 (MQ=255)                                                                   cG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGa                                                                                                 <  1:236961/106‑1 (MQ=255)                                                                   cG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGa                                                                                                 >  2:236961/1‑106 (MQ=255)                                                                          ggTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGAc                                                          >  1:626820/1‑139 (MQ=255)                                                                              gCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGc                                                      >  1:637859/1‑139 (MQ=255)                                                                                tcatcaTCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCtt                                                    >  2:339466/1‑139 (MQ=255)                                                                                  atcatcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCtt                                                                                        >  2:672126/1‑101 (MQ=255)                                                                                  atcatcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCtt                                                                                        <  1:672126/101‑1 (MQ=255)                                                                                               tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAAc                                      >  1:410324/1‑138 (MQ=255)                                                                                               tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAAc                                      >  2:250814/1‑138 (MQ=255)                                                                                               tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAAc                                      >  2:576718/1‑138 (MQ=255)                                                                                                   aGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCt                                 <  2:410324/139‑1 (MQ=255)                                                                                                     ggCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTgg                               >  2:265576/1‑139 (MQ=255)                                                                                                        cgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTa                                                                                                                      >  2:435618/1‑49 (MQ=255)                                                                                                        cgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTa                                                                                                                      <  1:435618/49‑1 (MQ=255)                                                                                                        cgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGa                            <  2:108488/139‑1 (MQ=255)                                                                                                               aGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACca                     <  1:265576/139‑1 (MQ=255)                                                                                                                        tGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCg            >  1:196557/1‑139 (MQ=255)                                                                                                                            aGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCt        <  2:544921/139‑1 (MQ=255)                                                                                                                             gCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCtt       >  1:302989/1‑139 (MQ=255)                                                                                                                             gCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCtt       >  1:529524/1‑139 (MQ=255)                                                                                                                                 ttAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCg   <  1:576718/139‑1 (MQ=255)                                                                                                                                   aGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGt  <  1:185923/138‑1 (MQ=255)                                                                                                                                   aGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGt  <  2:293075/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCG‑AACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGT  >  NZ_CP009273/1919687‑1919953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑