Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,948,189	T→G	V164G (GTG→GGG)	cmoB →	tRNA 5‑methoxyuridine(34)/uridine 5‑oxyacetic acid(34) synthase CmoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,948,189	0	T	G	100.0%	161.6 / NA	44	V164G (GTG→GGG)	cmoB	tRNA 5‑methoxyuridine(34)/uridine 5‑oxyacetic acid(34) synthase CmoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/23);  total (21/23)

TTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGA  >  NZ_CP009273/1948058‑1948318                                                                                                                                    |                                                                                                                                  tttAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGTTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTaaa                                                                                                                            <  2:771403/139‑1 (MQ=255)     aCCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAacta                                                                                                                         <  2:664243/138‑1 (MQ=255)      ccGGACGCTCCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                       <  1:514188/139‑1 (MQ=255)          aCGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAacta                                                                                                                         >  1:403899/1‑133 (MQ=255)          aCGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAacta                                                                                                                         <  2:403899/133‑1 (MQ=255)                       gATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAgcgcg                                                                                                      >  1:505774/1‑139 (MQ=255)                        aTGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAacta                                                                                                                         <  2:242802/119‑1 (MQ=255)                        aTGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAacta                                                                                                                         >  1:242802/1‑119 (MQ=255)                            cGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGCTCAGCGCGCGCAt                                                                                                 >  1:61145/1‑139 (MQ=255)                            cGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCAt                                                                                                 >  1:605212/1‑139 (MQ=255)                            cGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCAt                                                                                                 >  2:54070/1‑139 (MQ=255)                                 gCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                             >  2:107941/1‑138 (MQ=255)                                  cGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                             <  2:398306/137‑1 (MQ=255)                                             aTCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTa                                                                                 <  2:631928/138‑1 (MQ=255)                                                    gtgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTa                                                                                           >  1:278605/1‑121 (MQ=255)                                                    gtgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTa                                                                                           <  2:278605/121‑1 (MQ=255)                                                    gtgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGtt                                                                                      <  1:369414/126‑1 (MQ=255)                                                    gtgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGtt                                                                                      >  2:369414/1‑126 (MQ=255)                                                     tgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                             <  1:564714/118‑1 (MQ=255)                                                     tgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                             >  2:564714/1‑118 (MQ=255)                                                          cATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                       <  1:756560/87‑1 (MQ=255)                                                          cATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                       >  2:756560/1‑87 (MQ=255)                                                           aTGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCgg                                                                  <  1:107941/139‑1 (MQ=255)                                                            tGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTa                                                                                                                              >  1:362134/1‑78 (MQ=255)                                                            tGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTa                                                                                                                              <  2:362134/78‑1 (MQ=255)                                                            tGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCgg                                                                  >  2:301724/1‑138 (MQ=255)                                                              aTTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCAc                                                               >  2:132122/1‑139 (MQ=255)                                                                             cACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATa                                                >  1:730738/1‑139 (MQ=255)                                                                                         gggATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCg                                     <  1:132122/138‑1 (MQ=255)                                                                                         gggATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCg                                     <  2:605212/138‑1 (MQ=255)                                                                                             tCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATggg                                <  2:36578/139‑1 (MQ=255)                                                                                             tCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATggg                                <  1:86397/139‑1 (MQ=255)                                                                                               gACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCg                              <  2:160471/139‑1 (MQ=255)                                                                                                 cccTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTg                            >  2:396023/1‑139 (MQ=255)                                                                                                   cTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGa                                                            >  2:153685/1‑105 (MQ=255)                                                                                                   cTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGa                                                            <  1:153685/105‑1 (MQ=255)                                                                                                          aCTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCAt                   <  2:173833/139‑1 (MQ=255)                                                                                                           cTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATc                  >  1:222801/1‑139 (MQ=255)                                                                                                           cTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATc                  >  2:767004/1‑139 (MQ=255)                                                                                                              tttCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGtt                                                                                      >  1:208053/1‑68 (MQ=255)                                                                                                              tttCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGtt                                                                                      <  2:208053/68‑1 (MQ=255)                                                                                                                         gTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTg    >  1:180575/1‑139 (MQ=255)                                                                                                                            tGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGa  <  1:402303/138‑1 (MQ=255)                                                                                                                            tGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGa  <  1:54070/138‑1 (MQ=255)                                                                                                                                    |                                                                                                                                  TTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGA  >  NZ_CP009273/1948058‑1948318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑