| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,976,083 | T→G | A313A (GCA→GCC) | araH ← | arabinose ABC transporter permease AraH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,976,083 | 0 | T | G | 97.4% | 137.9 / ‑5.9 | 38 | A313A (GCA→GCC) | araH | arabinose ABC transporter permease AraH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (10/27); minor base C (1/0); total (11/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTT > NZ_CP009273/1975950‑1976214 | gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATTGTGGGGAAAAAAGCCAAATTTTTGCAGAAAAAAACATCAGACAGTGCGTTTCGCCTTTTGCTTTTTACGGGTGGAGATCACCCCCGCcag > 1:593993/1‑139 (MQ=255)gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCcag < 1:180476/139‑1 (MQ=255)gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCcag < 1:202706/139‑1 (MQ=255) cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTTGAAGATCACCGCGGCCAGCAGGATTAAGCcgc < 1:665496/139‑1 (MQ=255) cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc < 1:757393/139‑1 (MQ=255) cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc < 2:443005/139‑1 (MQ=255) cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc < 2:195700/139‑1 (MQ=255) cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc < 1:266210/139‑1 (MQ=255) aagaaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGa < 2:29380/139‑1 (MQ=255) aaCAGCCAACCGTTAGGGAGGTTGGCTATGGTGGGAAGAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAAcc < 1:600907/139‑1 (MQ=255) tGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaa < 1:618585/138‑1 (MQ=255) tGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaa < 1:547087/138‑1 (MQ=255) ggTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag < 2:94140/139‑1 (MQ=255) gCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAata < 1:376181/139‑1 (MQ=255) tggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCa < 2:314575/139‑1 (MQ=255) aaaaaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGtt > 2:723049/1‑139 (MQ=255) aaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGa < 1:22602/87‑1 (MQ=255) aaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGa > 2:22602/1‑87 (MQ=255) aaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATg < 1:342424/139‑1 (MQ=255) aCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag > 1:161789/1‑118 (MQ=255) aCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag < 2:161789/118‑1 (MQ=255) ttgCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACg > 2:663511/1‑139 (MQ=255) gAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGcc < 2:75819/139‑1 (MQ=255) aaaaaaGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCt < 1:98554/139‑1 (MQ=255) aGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGa < 2:114271/138‑1 (MQ=255) aGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag > 1:378970/1‑86 (MQ=255) aGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag < 2:378970/86‑1 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAGGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc > 2:357122/1‑139 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc > 1:599307/1‑60 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc < 2:599307/60‑1 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaa < 2:411941/79‑1 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaa > 1:411941/1‑79 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc > 1:534143/1‑139 (MQ=255) tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc < 1:271377/139‑1 (MQ=255) tttCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGccacc < 2:593993/139‑1 (MQ=255) tCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGa < 2:186607/36‑1 (MQ=255) tCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGa > 1:186607/1‑36 (MQ=255) tCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCttt < 1:663511/139‑1 (MQ=255) | GTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTT > NZ_CP009273/1975950‑1976214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |