Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,980,983	T→G	intergenic (+74/+5)	ftnB → / ← yecJ	non‑heme ferritin‑like protein/DUF2766 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,980,983	0	T	G	100.0%	159.3 / NA	44	intergenic (+74/+5)	ftnB/yecJ	non‑heme ferritin‑like protein/DUF2766 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/20);  total (24/20)

TGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACA  >  NZ_CP009273/1980853‑1981118                                                                                                                                   |                                                                                                                                        tgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTa                                                                                                                                  <  1:135260/138‑1 (MQ=255)        tgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGAt                                                                                                                           <  1:577827/138‑1 (MQ=255)               cgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCtg                                                                                                                   >  1:717704/1‑139 (MQ=255)                  ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGCTTCACCCTGTgg                                                                                                                >  2:337903/1‑139 (MQ=255)                  ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTgg                                                                                                                >  1:743350/1‑139 (MQ=255)                                    cGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCaa                                                                                               >  1:293953/1‑138 (MQ=255)                                           cacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgtgc                                                                                        >  1:533087/1‑138 (MQ=255)                                                    cATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtt                                                                                <  2:293953/137‑1 (MQ=255)                                                        gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGa                                                                          >  2:592797/1‑139 (MQ=255)                                                         atcatcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCTTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGAt                                                                         <  1:207155/139‑1 (MQ=255)                                                          tcatcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGAtt                                                                        >  1:293440/1‑139 (MQ=255)                                                          tcatcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGAtt                                                                        >  1:61598/1‑139 (MQ=255)                                                            atcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGc                                                                      >  1:482218/1‑139 (MQ=255)                                                              catcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGccc                                                                    >  2:459724/1‑139 (MQ=255)                                                              catcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGccc                                                                    >  2:527664/1‑139 (MQ=255)                                                                  ggCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCa                                                                 <  1:572159/138‑1 (MQ=255)                                                                   gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                                                                    >  1:281458/1‑102 (MQ=255)                                                                   gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                                                                    <  2:281458/102‑1 (MQ=255)                                                                        aaTGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAc                                                          >  2:407623/1‑139 (MQ=255)                                                                               tGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTc                                                                                                                         >  1:59598/1‑69 (MQ=255)                                                                               tGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTc                                                                                                                         <  2:59598/69‑1 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAAt                                                                                                                                <  2:50715/60‑1 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAAt                                                                                                                                >  1:50715/1‑60 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTc                                                                                                                         >  1:285688/1‑67 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTc                                                                                                                         <  2:285688/67‑1 (MQ=255)                                                                                      aTGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcggcgg                                            >  1:627467/1‑139 (MQ=255)                                                                                          aGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGTGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCggg                                        <  2:533087/139‑1 (MQ=255)                                                                                          aGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCggg                                        <  2:627467/139‑1 (MQ=255)                                                                                          aGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCggg                                        <  2:247799/139‑1 (MQ=255)                                                                                          aGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCggg                                        <  1:704990/139‑1 (MQ=255)                                                                                          aGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCggg                                        <  1:527664/139‑1 (MQ=255)                                                                                           ggTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGa                                       >  2:217487/1‑139 (MQ=255)                                                                                            gTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGAt                                      <  1:762015/139‑1 (MQ=255)                                                                                                    aaaCCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTa                              <  1:592797/139‑1 (MQ=255)                                                                                                       ccGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAg                           >  2:455086/1‑139 (MQ=255)                                                                                                        cGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGt                          <  1:217487/139‑1 (MQ=255)                                                                                                             ccTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGata                     >  1:430424/1‑139 (MQ=255)                                                                                                             ccTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGa                       <  2:293440/137‑1 (MQ=255)                                                                                                             ccTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGa                       <  2:61598/137‑1 (MQ=255)                                                                                                                  tCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGt                          >  2:178345/1‑129 (MQ=255)                                                                                                                  tCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGt                          <  1:178345/129‑1 (MQ=255)                                                                                                                  tCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATAttctt                >  2:254765/1‑139 (MQ=255)                                                                                                                                cGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGTGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACa  >  1:443603/1‑139 (MQ=255)                                                                                                                                cGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACa  >  1:772093/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                        TGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACA  >  NZ_CP009273/1980853‑1981118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑