Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,982,130	G→A	intergenic (+104/‑67)	yecR → / → ftnA	YecR‑like lipofamily protein/non‑heme ferritin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,982,130	0	G	A	100.0%	96.7 / NA	25	intergenic (+104/‑67)	yecR/ftnA	YecR‑like lipofamily protein/non‑heme ferritin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/17);  total (8/17)

GCCATCATTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTACTCTTCA  >  NZ_CP009273/1981995‑1982259                                                                                                                                        |                                                                                                                                  gCCATCATTTCGCCTTCCCTGTCTTTTTCTAAATATTTTAATCAGCGAGGGTTTCTTCGCTGATTAAAGAAATAGCGGTAATTTGTTTTATTCCTATTCGTATTGTTAATAATTGAAGTTTATATTTTACCTTTTACa                                                                                                                                 >  2:408386/1‑138 (MQ=255) gCCATCATTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACa                                                                                                                                 >  1:68215/1‑138 (MQ=255)     tcatTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAaataa                                                                                                                            >  2:80717/1‑139 (MQ=255)     tcatTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAaataa                                                                                                                            <  2:68215/139‑1 (MQ=255)            gCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATaataaaata                                                                                                                     <  2:24265/139‑1 (MQ=255)              cTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAAc                                                                                                                   <  2:743352/139‑1 (MQ=255)                                     tttAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaa                                                                                            >  2:46183/1‑139 (MQ=255)                                      ttAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaa                                                                                            <  2:756627/138‑1 (MQ=255)                                       tAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaac                                                                                           <  2:339481/138‑1 (MQ=255)                                        aaTCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCcaca                                                                                                >  1:374824/1‑132 (MQ=255)                                        aaTCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCcaca                                                                                                <  2:374824/132‑1 (MQ=255)                                            aGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTGTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAatat                                                                                     <  1:757743/139‑1 (MQ=255)                                              cGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaa                                                                                            <  1:555255/130‑1 (MQ=255)                                              cGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaa                                                                                            >  2:555255/1‑130 (MQ=255)                                               gAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAAc                                                                                  <  1:240486/139‑1 (MQ=255)                                                        ttCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTtgtg                                                                           >  1:146358/1‑137 (MQ=255)                                                          cGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAg                                                                        <  2:339299/138‑1 (MQ=255)                                                               aTTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTAt                                                                  <  1:58428/139‑1 (MQ=255)                                                                     gAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCt                                                            <  1:80717/139‑1 (MQ=255)                                                                                      ttttATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGAtt                                            >  2:237972/1‑138 (MQ=255)                                                                                                              taatTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAAcc                   <  1:271896/139‑1 (MQ=255)                                                                                                                    aaGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGaa              <  2:489567/138‑1 (MQ=255)                                                                                                                    aaGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGaa              <  1:174092/138‑1 (MQ=255)                                                                                                                         tataTTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTa         <  1:46183/138‑1 (MQ=255)                                                                                                                                tACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTACTCTTCa  <  2:427737/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GCCATCATTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTACTCTTCA  >  NZ_CP009273/1981995‑1982259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑