Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	75,122	G→A	A339T (GCA→ACA)	setA →	sugar efflux transporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	75,122	0	G	A	100.0%	138.4 / NA	34	A339T (GCA→ACA)	setA	sugar efflux transporter SetA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

TACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTA  >  NZ_CP009273/74990‑75256                                                                                                                                     |                                                                                                                                       tACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTa                                                                                                                                  >  2:87860/1‑139 (MQ=255)       ggATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCt                                                                                                                            <  1:450110/139‑1 (MQ=255)        gattgattTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCt                                                                                                                            >  1:173811/1‑138 (MQ=255)        gattgattTTCTTTAATAGCCGTATGGCGTTGATGAAGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTaccacat                                                                                                                            >  2:80897/1‑136 (MQ=255)               tttCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTa                                                                                                                          <  2:573510/133‑1 (MQ=255)               tttCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTa                                                                                                                          >  1:573510/1‑133 (MQ=255)                     tAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAAc                                                                                                                 <  1:769289/136‑1 (MQ=255)                             gTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                      <  2:418960/139‑1 (MQ=255)                             gTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                      <  2:46225/139‑1 (MQ=255)                                  gCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCgggg                                                                                                 <  1:96512/139‑1 (MQ=255)                                     ttGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTaa                                                                                              >  1:301099/1‑139 (MQ=255)                                        atgaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTGACAGTATTTCTACCGGGGTAATTc                                                                                           >  2:2876/1‑139 (MQ=255)                                             gCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACt                                                                                                                    >  1:384403/1‑109 (MQ=255)                                             gCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACt                                                                                                                    <  2:384403/109‑1 (MQ=255)                                                    cTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTGACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTa                                                                               <  1:2876/139‑1 (MQ=255)                                                         tAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCa                                                                           <  1:627975/138‑1 (MQ=255)                                                                 tATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAAt                                                                  <  2:301099/139‑1 (MQ=255)                                                                      aTCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGca                                                              >  1:589934/1‑138 (MQ=255)                                                                           cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                        >  1:112777/1‑139 (MQ=255)                                                                           cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                        >  1:721840/1‑139 (MQ=255)                                                                           cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                        >  1:656918/1‑139 (MQ=255)                                                                           cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                        >  1:150898/1‑139 (MQ=255)                                                                                ttgCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTgggg                                                   <  2:64194/139‑1 (MQ=255)                                                                                     ggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                              >  2:691169/1‑139 (MQ=255)                                                                                     ggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                              >  1:103745/1‑139 (MQ=255)                                                                                           gggATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                         <  1:87860/138‑1 (MQ=255)                                                                                                     ggTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCt                                                                                                                            <  2:197747/45‑1 (MQ=255)                                                                                                     ggTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCt                                                                                                                            >  1:197747/1‑45 (MQ=255)                                                                                                           aGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCa                                                <  1:574758/115‑1 (MQ=255)                                                                                                           aGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCa                                                >  2:574758/1‑115 (MQ=255)                                                                                                           aGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGc                        >  2:711854/1‑139 (MQ=255)                                                                                                            ggATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                       <  1:718761/139‑1 (MQ=255)                                                                                                                        tGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgtt           >  1:424780/1‑139 (MQ=255)                                                                                                                                cGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCat    >  1:527823/1‑138 (MQ=255)                                                                                                                                 ggggaCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCatta  >  1:270621/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       TACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTA  >  NZ_CP009273/74990‑75256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑