Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,052,030	T→C	D433G (GAT→GGT)	yeeO ←	toxic metabolite efflux MATE transporter YeeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,052,030	0	T	C	100.0%	163.3 / NA	46	D433G (GAT→GGT)	yeeO	toxic metabolite efflux MATE transporter YeeO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/19);  total (27/19)

ATCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACC  >  NZ_CP009273/2051895‑2052165                                                                                                                                        |                                                                                                                                        aTCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCt                                                                                                                                      <  2:652148/139‑1 (MQ=255)   cGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTgg                                                                                                                                    >  2:527095/1‑139 (MQ=255)              tGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGa                                                                                                                         <  1:495963/139‑1 (MQ=255)                 cGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGc                                                                                                                      >  2:586845/1‑139 (MQ=255)                        ggCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAAcc                                                                                                               <  1:577439/139‑1 (MQ=255)                        ggCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAAcc                                                                                                               <  1:423316/139‑1 (MQ=255)                           aTCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCg                                                                                                            >  2:137829/1‑139 (MQ=255)                           aTCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCg                                                                                                            >  1:224304/1‑139 (MQ=255)                           aTCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCg                                                                                                            >  1:596399/1‑139 (MQ=255)                                  gCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAgg                                                                                                     <  2:692044/139‑1 (MQ=255)                                                aGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAg                                                                                       >  2:398529/1‑139 (MQ=255)                                                aGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAg                                                                                       >  1:177872/1‑139 (MQ=255)                                                 gCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAgg                                                                                      <  2:596399/139‑1 (MQ=255)                                                  cGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGc                                                                                     >  2:571683/1‑139 (MQ=255)                                                               tGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAAt                                                                        >  1:704553/1‑139 (MQ=255)                                                                   gCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATcc                                                                      >  1:477729/1‑137 (MQ=255)                                                                   gCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATcc                                                                      >  1:334893/1‑137 (MQ=255)                                                                      gACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAAcc                                                                                                               >  1:341226/1‑93 (MQ=255)                                                                      gACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAAcc                                                                                                               <  2:341226/93‑1 (MQ=255)                                                                       aCCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGt                                                                >  1:603694/1‑139 (MQ=255)                                                                         cAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTgg                                                              <  1:586845/139‑1 (MQ=255)                                                                         cAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTgg                                                              <  2:232275/139‑1 (MQ=255)                                                                          aaaTAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGa                                                             <  1:156394/139‑1 (MQ=255)                                                                            aTAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGa                                                             >  2:156394/1‑137 (MQ=255)                                                                                gCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGc                                                         >  1:646320/1‑137 (MQ=255)                                                                                       taaaGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAAcaca                                                <  2:177872/139‑1 (MQ=255)                                                                                          aGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGc                                             <  2:162994/139‑1 (MQ=255)                                                                                          aGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGc                                             >  2:339700/1‑139 (MQ=255)                                                                                               ttAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCaa                                        >  1:508621/1‑139 (MQ=255)                                                                                                       aTCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAAtct                                >  2:223176/1‑139 (MQ=255)                                                                                                              tCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTg                         <  2:459126/139‑1 (MQ=255)                                                                                                                     gACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATc                  <  1:294112/139‑1 (MQ=255)                                                                                                                      aCATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGt                                                                            >  1:224075/1‑80 (MQ=255)                                                                                                                      aCATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGt                                                                            <  2:224075/80‑1 (MQ=255)                                                                                                                      aCATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCt                 >  1:641245/1‑139 (MQ=255)                                                                                                                           tttAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGccc             <  2:646320/138‑1 (MQ=255)                                                                                                                              aaCCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGTGAGCCGTTAGCCAGGCGATgg                                                                                <  2:383900/68‑1 (MQ=255)                                                                                                                              aaCCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATgg                                                                                >  1:383900/1‑68 (MQ=255)                                                                                                                                 cTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTAccccc      <  1:339700/139‑1 (MQ=255)                                                                                                                                    tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAAc   >  1:156052/1‑139 (MQ=255)                                                                                                                                    tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAAc   >  1:704800/1‑139 (MQ=255)                                                                                                                                    tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAAc   >  2:593805/1‑139 (MQ=255)                                                                                                                                    tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAAc   >  1:92481/1‑139 (MQ=255)                                                                                                                                     ggACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAggg                                                                                                    >  1:288496/1‑41 (MQ=255)                                                                                                                                     ggACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAggg                                                                                                    <  2:288496/41‑1 (MQ=255)                                                                                                                                     ggACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAAcc  >  1:420218/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        ATCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACC  >  NZ_CP009273/2051895‑2052165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑