Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,065,124	T→G	G35G (GGT→GGG)	agn43 →	autotransporter adhesin Ag43

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,065,124	0	T	G	100.0%	67.6 / NA	19	G35G (GGT→GGG)	agn43	autotransporter adhesin Ag43
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/9);  total (10/9)

TTTTATTCACCCTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAC  >  NZ_CP009273/2064989‑2065258                                                                                                                                        |                                                                                                                                       ttttAGTCACCCTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACgtggcggggt                                                                                                                                      <  1:366706/138‑1 (MQ=255)                     aGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCCCTGTCTCTTGc                                                                                                                 >  1:233247/1‑139 (MQ=255)                     aGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGc                                                                                                                 >  1:489382/1‑139 (MQ=255)                           aGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACgtggcggggt                                                                                                                                      >  1:8125/1‑112 (MQ=255)                           aGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACgtggcggggt                                                                                                                                      <  2:8125/112‑1 (MQ=255)                                      cGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTcc                                                                                                <  2:524705/139‑1 (MQ=255)                                          aTCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGGTGGACTGTCTCTTGCCGCCGTCACGTCACTCCCggg                                                                                            >  2:29558/1‑138 (MQ=255)                                                    cTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGgctgct                                                                                  <  1:309031/139‑1 (MQ=255)                                                    cTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGgctgct                                                                                  >  1:715823/1‑139 (MQ=255)                                                      gCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGGGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGa                                                                                >  1:176486/1‑139 (MQ=255)                                                           aGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCg                                                                           <  1:147956/139‑1 (MQ=255)                                                             gCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACgtggcggggtggcgg                                                                                                                                 >  1:3175/1‑83 (MQ=255)                                                             gCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACgtggcggggtggcgg                                                                                                                                 <  2:3175/83‑1 (MQ=255)                                                              cTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTtg                                                                        >  2:138417/1‑139 (MQ=255)                                                                 gTATGGAATCACATGGCGGGCGCTTTCGTGGTTGCCTCCGGACTGGCCCGCGCACGGGGTAAACGGGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTtgtg                                                                      <  1:650367/138‑1 (MQ=255)                                                                 gTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTtgtg                                                                      >  1:388350/1‑138 (MQ=255)                                                                           acaTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCa                                                                                                                            <  1:67114/74‑1 (MQ=255)                                                                           acaTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCa                                                                                                                            >  2:67114/1‑74 (MQ=255)                                                                           acaTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGgag                                                            <  2:489382/138‑1 (MQ=255)                                                                                 cGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCTCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCgg                                                     >  1:404367/1‑138 (MQ=255)                                                                                    gcgcTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGaa                                                  <  2:233247/139‑1 (MQ=255)                                                                                    gcgcTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGaa                                                  <  2:388350/139‑1 (MQ=255)                                                                                                                ccGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAAc                      <  1:138417/139‑1 (MQ=255)                                                                                                                              aaCGTGGCGGGGTGGCGGTTTCCCTGTCTCTTGCCGCCGTCCCGTCACTCCCGGTGCTGGCTTCTGGCCTCGTTGTCCCCCCCGGAGAAACCGTGAACGGCGGGACCCTTGCCAATCCTGACCACCCGATTTTCTTCgg        >  2:188569/1‑139 (MQ=255)                                                                                                                                    gcggggtggcggtTTCACTGTCTCTTGCCGCCGTCACGTCACTCCCGGTGCTGGCCGCTGACCTTGTTGTGCACCCCGGGGAAAACGCGGAAGGCGGAACCATGGCCAATCATGGCAAACCGATTGTCTTCGGTACGaa  >  2:424352/1‑138 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTTTGCACCCGGGGGAAACCGTGAAAGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAc  >  2:665787/1‑139 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGaa                                                  >  1:239034/1‑91 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGaa                                                  <  2:239034/91‑1 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCAt                            >  1:770913/1‑113 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCAt                            <  2:770913/113‑1 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGt       <  1:79985/134‑1 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGt       >  2:79985/1‑134 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAc  >  1:395373/1‑139 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAc  >  1:537800/1‑139 (MQ=255)                                                                                                                                    gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGGGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGGACGGCGGGACACTGGCAAATCATGACCACCAGATTGTCTTCGGTACGAc  >  2:635873/1‑139 (MQ=255)                                                                                                                                    gcgggggggcggtTGCACTGTCTCTTGCCGCAGTTACGTCACTCCCGGGGCTGGCTGCTGACATCGTTGTGCACCCGGGGGAAACCGTGTACGGCGGAAAACTGGCAAATCATGACAACCAGGTTGGCTTCGGgacgac  >  2:446791/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       TTTTATTCACCCTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAC  >  NZ_CP009273/2064989‑2065258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑