Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,071,153	G→C	G35A (GGC→GCC)	cbtA →	type IV toxin‑antitoxin system cytoskeleton‑binding toxin CbtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,071,153	0	G	C	100.0%	96.0 / NA	27	G35A (GGC→GCC)	cbtA	type IV toxin‑antitoxin system cytoskeleton‑binding toxin CbtA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/17);  total (10/17)

TATCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACC  >  NZ_CP009273/2071023‑2071278                                                                                                                                   |                                                                                                                              tatCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTcaca                                                                                                                        <  2:41210/138‑1 (MQ=255)   tCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACt                                                                                                                      <  2:251344/138‑1 (MQ=255)       cATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAAt                                                                                                                  >  1:70722/1‑138 (MQ=255)                        cAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGa                                                                                                 <  2:91114/138‑1 (MQ=255)                             tGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACg                                                                                           <  1:487201/139‑1 (MQ=255)                                    aTTACCTGTAGTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                                                                                    <  2:348348/139‑1 (MQ=255)                                     ttACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                                                                                    >  1:549239/1‑138 (MQ=255)                                       aCCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGa                                                                                  <  2:737620/138‑1 (MQ=255)                                        ccTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag                                                                                 <  2:195394/138‑1 (MQ=255)                                                aCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATAtt                                                                        >  1:80751/1‑139 (MQ=255)                                                     ggCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggc                                                                   <  2:549239/139‑1 (MQ=255)                                                      gCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGagg                                                                    >  1:186745/1‑137 (MQ=255)                                                      gCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGagg                                                                    >  1:509568/1‑137 (MQ=255)                                                       cAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggc                                                                   <  2:509568/137‑1 (MQ=255)                                                               cAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                                                         <  1:579131/139‑1 (MQ=255)                                                                            tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGc                                                                                                    >  2:289453/1‑83 (MQ=255)                                                                            tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGc                                                                                                    <  1:289453/83‑1 (MQ=255)                                                                            tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtgtg                                                  <  1:598958/133‑1 (MQ=255)                                                                            tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtgtg                                                  >  2:598958/1‑133 (MQ=255)                                                                            tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCgg                                            >  1:596888/1‑139 (MQ=255)                                                                                             gCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGt                               <  1:450750/135‑1 (MQ=255)                                                                                                 aTACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAAt                       <  2:122062/139‑1 (MQ=255)                                                                                                       ctgTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATAcgcg                  <  2:186745/138‑1 (MQ=255)                                                                                                             cGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGc           >  2:96878/1‑139 (MQ=255)                                                                                                                      gACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCg                                             <  2:574982/96‑1 (MQ=255)                                                                                                                      gACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCg                                             >  1:574982/1‑96 (MQ=255)                                                                                                                      gACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGAcc  <  1:96878/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                              TATCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACC  >  NZ_CP009273/2071023‑2071278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑